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MilliporeSigma

L0144

Sigma-Aldrich

Lucifer Yellow CH dipotassium salt

powder

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$124.45
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$393.00

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25 MG
$124.45
100 MG
$393.00

About This Item

Empirical Formula (Hill Notation):
C13H9K2N5O9S2
CAS Number:
Molecular Weight:
521.57
Beilstein/REAXYS Number:
5224942
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.77

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$124.45

List Price$131.00
Web-Only Promotion

In StockDetails


Request a Bulk Order
Technical Service
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form

powder

color

orange

fluorescence

λex 428 nm; λem 540 nm

storage temp.

room temp

SMILES string

[K+].[K+].NNC(=O)NN1C(=O)c2cc(cc3c(N)c(cc(C1=O)c23)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C13H11N5O9S2.2K/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2

InChI key

HCWYSCVNSCIZCN-UHFFFAOYSA-L

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1 of 4

This Item
L0259L351050227
form

powder

form

powder

form

powder

form

powder or crystals

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

room temp

storage temp.

2-8°C

storage temp.

room temp

storage temp.

−20°C

color

orange

color

faint orange to very dark orange

color

yellow to brown

color

-

fluorescence

λex 428 nm; λem 540 nm

fluorescence

λex 428 nm; λem 540 nm in H2O

fluorescence

λex 428 nm; λem 536 nm in H2O

fluorescence

-

General description

Lucifer yellow CH is a fixable dye with an excitation and emission spectrum of 430 nm and 540 nm, respectively.[1]LuciferYellow CH (LYCH) is a small, hydrophilic molecule,[2] and highly fluorescent dye that is used for intracellular staining for the studies conducted on various types of cells.[3] It acts as a marker for the detection of well-established tight junctions[2] in smooth muscles, glia, and interstitial cells of Cajal (ICC).[3]

Application

Lucifer yellow CH dipotassium salt has been used:
  • to visualize cell morphology[1]
  • to assess intercellular communication activity through gap junction by scrape-loading assay[4][5]
  • as a component of the intracellular solution, which filled the dendrites for the visualization of neuronal morphology[6]
  • in the intracellular solution, to intracellularly label recorded neurons[7]

Features and Benefits

Highly fluorescent dye, useful in marking nerve cells.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Questions

1–2 of 2 Questions  
  1. What is the dye purity for product L0144?

    What is the dye purity for product L0144?

    1 answer
    1. At M-Clarity quality level 100, Product L0144 is evaluated based on appearance, infrared spectrum, solubility, and fluorescence specifications. Please refer to the Certificate of Analysis found on the DOCUMENTATION section of the Product Detail Page: https://www.sigmaaldrich.com/product/sigma/l0144#product-documentation

      Using the results on the specification sheet, dye content can be calculated from the testing performed to determine carbon and nitrogen levels.

      Helpful?

  2. What is the stability and shelf life for product L0144, lot SHBN6250 once it has been prepared in a solution?

    1 answer
    1. The product is soluble in water at a concentration of 1 mg/mL, producing a clear, yellow-orange colored solution. It is also soluble in water with heat at a concentration of 10 mg/mL, resulting in a slightly hazy orange colored solution. Additionally, a solution stability test revealed that a solution of L0144 in water at a concentration of 1.7 mg/mL, stored in the dark at room temperature for 32 days, showed no detectable change in the UV-VIS spectrum.

      Helpful?

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