There is no specific protocol associated with this product, however our GenElute Bacterial Genomic DNA kits ( NA2100, NA2110, NA2120) include a lysis protocol specific to Staphylococcus species that may be helpful. In this protocol, Lysostaphin is used at 200 units per ml in combination with mutanolysin and a lysis buffer included in the kit. See the link below, page 6 to review this information.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/256/042/na2100bul.pdf
L9043
Lysostaphin from Staphylococcus simulans
recombinant, expressed in E. coli, lyophilized powder
Synonym(s):
Glycyl-glycine Endopeptidase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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biological source
bacterial
recombinant
expressed in E. coli
assay
≥90% (HPLC)
form
lyophilized powder
specific activity
≥3000 unit/mg solid
technique(s)
molecular cloning: suitable
suitability
suitable for sequence analysis test
application(s)
life science and biopharma
shipped in
dry ice
storage temp.
−20°C
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This Item | SRE0053 | L2898 | SAE0091 |
|---|---|---|---|
| biological source bacterial | biological source bacterial (Staphylococcus sp.) | biological source - | biological source microbial (Staphylococcus staphylotyticus) |
| technique(s) molecular cloning: suitable | technique(s) - | technique(s) - | technique(s) - |
| assay ≥90% (HPLC) | assay - | assay - | assay - |
| specific activity ≥3000 unit/mg solid | specific activity ≥500 units/mg protein | specific activity >500 units/mg protein | specific activity ≥500 units/mg protein |
| suitability suitable for sequence analysis test | suitability suitable for microbiological tests | suitability - | suitability - |
| form lyophilized powder | form lyophilized powder | form powder | form lyophilized powder |
General description
Lysostaphin, a zinc metalloenzyme, is mainly secreted as a proenzyme. It acts as an anti-staphylococcal agent and is highly stable at pH 4 and 5°C.[1]
Application
Lysostaphin from Staphylococcus simulans has been used in a study to assess molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus. [2] It has also been used in a study to investigate the sequence analysis of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity. [3]
Biochem/physiol Actions
A protein complex with highly specific lytic activity against Staphylococcus species, including Staphylococcus aureus.
Lysostaphin possesses a lytic action against Staphylococcus aureus, which is used to treat antibiotic-resistant staphylococcal infections. It has the properties of enzymes, such as glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acetyl muramyl-L-alanine amidase. Lysostaphin is also used as a preservative in food industry and in clinical labs for rapid screening.[1]
The antimicrobial activity of lysostaphin, a metalloendopeptidase expressed by Staphylococcus simulans, is enhanced by binding to the C′ terminus of the membrane-associated Trap protein of Staphylococcus aureus. [4]
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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C-terminus of TRAP in Staphylococcus can enhance the activity of lysozyme and lysostaphin.
Yang, G.
Acta Biochimica et Biophysica Hungarica, 40, 452-458 (2008)
L Ramadurai et al.
Journal of bacteriology, 179(11), 3625-3631 (1997-06-01)
A gene encoding an autolytic activity was identified in an autolysis-deficient mutant (Lyt-) of Staphylococcus aureus which produces only a single band in autolytic-activity gels (N. Mani, P. Tobin, and R. K. Jayaswal, J. Bacteriol. 175:1493-1499, 1993). An open reading
X Wang et al.
Gene, 102(1), 105-109 (1991-06-15)
The nucleotide (nt) sequence of a 2.0-kb NheI-XbaI DNA fragment containing a peptidoglycan hydrolase-encoding gene, lytA, tentatively identified as encoding an N-acetylmuramyl-L-alanine amidase, from Staphylococcus aureus, was determined. The nt sequencing revealed an open reading frame (ORF) of 1443 bp
Lysostaphin: an antistaphylococcal agent
Kumar J K
Applied Microbiology and Biotechnology, 80(4), 555-561 (2008)
Grégory Francius et al.
Journal of bacteriology, 190(24), 7904-7909 (2008-10-07)
The advent of Staphylococcus aureus strains that are resistant to virtually all antibiotics has increased the need for new antistaphylococcal agents. An example of such a potential therapeutic is lysostaphin, an enzyme that specifically cleaves the S. aureus peptidoglycan, thereby
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Is there a standard protocol from the manufacturer on how to lyse Staphylococcus aureus cells? What concentration, temperature, time ...
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How should a stock solution of the Lysostaphin from Staphylococcus simulans enzyme, product L9043, be prepared?
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A stock solution of this recombinant, Lysostaphin from Staphylococcus simulans, may be prepared by reconstituting it at 10 mg/ml in 20 mM sodium acetate buffer, pH 4.5 and storing it aseptically at 4°C. in a polypropylene container.
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