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MOD50

Sigma-Aldrich

Imprint® DNA Modification Kit

For bisulfite DNA conversion & purification

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Synonym(s):
Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification
EC Number:
NACRES:
NA.54

usage

sufficient for 50 reactions

storage temp.

20-25°C

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This Item
RIPNA0100MSNU030
storage temp.

20-25°C

storage temp.

-

storage temp.

15-25°C

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

400

General description

The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.

Application

Imprint® DNA Modification Kit has been used for bisulfite modification of DNA.

Features and Benefits

  • Only 50 picograms of DNA or 20 cells are required
  • Procedure takes less than 2 hours
  • Greater than 99% conversion rate
  • Extremely low degradation
  • Option of convenient one-step protocol
  • Consistent and reproducible Bisulfite Modification
  • Can be used with genomic, endonuclease digested, and FFPE DNA

Storage and Stability

All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS

  • T3566Clear-view Snap-Cap microtubes, size 1.5 mL, naturalSDS

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Supplementary Hazards

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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The elephant shark methylome reveals conservation of epigenetic regulation across jawed vertebrates
Peat JR, et al.
F1000Research (2017)
Sarah Rose Huffman et al.
Methods in molecular biology (Clifton, N.J.), 925, 201-209 (2012-08-22)
Researchers whose experimental models are mammalian oocytes and preimplantation embryos are often limited by the yield of nucleic acids that can be isolated from such a small sample size. In addition, the limited number of cells from these types of
Julian R Peat et al.
Methods in molecular biology (Clifton, N.J.), 1708, 161-169 (2017-12-11)
The epigenetic mark 5-methylcytosine confers heritable regulation of gene expression that can be dynamically modulated during transitions in cell fate. With the development of high-throughput sequencing technologies, it is now possible to obtain comprehensive genome-wide maps of the mammalian DNA
M Y Follo et al.
Leukemia, 26(5), 943-950 (2011-10-29)
Phosphoinositide-phospholipase C (PI-PLC) beta1 can be considered a specific target for demethylating therapy in high-risk myelodysplastic syndrome (MDS) patients, as azacitidine treatment has been associated with a PI-PLCbeta1-specific promoter demethylation, and induction of PI-PLCbeta1 gene and protein expression. However, little
Javed Hussain Choudhury et al.
Methods in molecular biology (Clifton, N.J.), 1726, 111-122 (2018-02-23)
DNA methylation plays a decisive role in the regulation and control of gene expression. DNA methylation is a covalent modification, in which a methyl group is attached to the 5th carbon of the cytosine ring of a CpG dinucleotide that

Articles

There are several common ways to determine whether a gene contains methylated DNA. Since mammalian methylation occurs at cytosines, researchers take advantage of the fact that methylated cytosine (meC) is stable to bisulfite treatment but unmethylated cytosine is transformed to uracil under the same conditions.

Related Content

The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service