MSRT1

MS RT Calibration Mix

Proteomics Retention Time Standard for LC-MS

• Synonym(s): Proteomics Retention Time Standard for LC-MS
• NACRES: NA.24

Properties

• quality: Proteomics Retention Time Standard for LC-MS
• Quality Level: 200
• analyte chemical class(es): amino acids, peptides, proteins
• technique(s): HPLC: suitable; LC/MS: suitable
• application(s): food and beverages
• format: multi-component solution
• storage temp.: −20°C

Description

General description

MSRT1 is an injection-ready standard, and can serve for quick tests of LC-MS platform performance, comparison of LC gradients and columns, monitoring column and system changes. MSRT1 can be used to predict retention times of peptides across LC-MS platforms. MSRT1 is also applicable to multiple reaction monitoring / selected reaction monitoring (MRM / SRM) analysis.

Components:
Each vial contains various amounts of 14 isotopically labeled synthetic peptides dried via vacuum centrifugation. Each peptide is labeled with either a [¹³C₆,¹⁵N₂] Lysine (+8 Da), a [¹³C₆,¹⁵N₄] Arginine (+10 Da), or a [¹³C₆,¹⁵N] Leucine (+7 Da).

Application

MS RT Calibration Mix has been used as a reference for iRT (relative retention times) calculations after LC-MS (liquid chromatography–mass spectrometry)/MS analysis.[1] It has been used to check the reliability and specificity of ETD-HCD (electron transfer dissociation-high-energy collisional dissociation) MS³ (mass spectrometry) method for leucine/isoleucine discrimination.[2]

The MS RT Calibration Mix is a LC-MS platform standard intended to test properties such as LC resolution, peptide elution profiles, and retention time prediction. The peptides in MSRT1 are designed to span the normal elution profile of complex proteomic samples. The peptides have been added in various amounts, based on electrospray response, to have relatively similar response and be readily observed.

MSRT1 is an injection-ready standard, and can serve for quick tests of LC-MS platform performance, comparison of LC gradients and columns, monitoring column and system changes. MSRT1 can be used to predict retention times of peptides across LC-MS platforms. MSRT1 is also applicable to multiple reaction monitoring / selected reaction monitoring (MRM / SRM) analysis.

Features and Benefits

General

Complexity

• Defined mixture gives confidence in your instruments analysis

Easily visualized

• Distinct LC-MS peak profile of peptides, readily separated with similar responses
• iRT Table (xls)

Injection-ready

• Decreases prep time

Qualitative:

• Defined mixture of 14 stable isotope labeled (SIL) peptides

Quantitative:
MRM settings provided (Skyline, xls)

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Legal Information

This product is licensed under U.S. Patent No. 7,396,688 and foreign counterparts from E. I. du Pont de Nemours and Company. The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product for research and development only, including services for a third party for consideration. The buyer cannot sell or otherwise transfer this product, its components or materials made using this product or its components to a third party. Information about licenses for excluded uses is available from: E. I. du Pont de Nemours and Company; Attn: Associate Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881-9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable