MS RT Calibration Mix

Proteomics Retention Time Standard for LC-MS


Quality Level


Proteomics Retention Time Standard for LC-MS

analyte chemical class(es)

amino acids, peptides, proteins


HPLC: suitable
LC/MS: suitable

Featured Industry

Food and Beverages


multi-component solution

storage temp.


General description

MSRT1 is an injection-ready standard, and can serve for quick tests of LC-MS platform performance, comparison of LC gradients and columns, monitoring column and system changes. MSRT1 can be used to predict retention times of peptides across LC-MS platforms. MSRT1 is also applicable to multiple reaction monitoring / selected reaction monitoring (MRM / SRM) analysis.

Each vial contains various amounts of 14 isotopically labeled synthetic peptides dried via vacuum centrifugation. Each peptide is labeled with either a [13C6,15N2] Lysine (+8 Da), a [13C6,15N4] Arginine (+10 Da), or a [13C6,15N] Leucine (+7 Da).


MS RT Calibration Mix has been used as a reference for iRT (relative retention times) calculations after LC-MS (liquid chromatography–mass spectrometry)/MS analysis. It has been used to check the reliability and specificity of ETD-HCD (electron transfer dissociation-high-energy collisional dissociation) MS3 (mass spectrometry) method for leucine/isoleucine discrimination.

Features and Benefits


  • Defined mixture gives confidence in your instruments analysis

Easily visualized
  • Distinct LC-MS peak profile of peptides, readily separated with similar responses
  • iRT Table (
  • Decreases prep time

  • Defined mixture of 14 stable isotope labeled (SIL) peptides

MRM settings provided (
Skyline, xls)

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Legal Information

This product is licensed under U.S. Patent No. 7,396,688 and foreign counterparts from E. I. du Pont de Nemours and Company. The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product for research and development only, including services for a third party for consideration. The buyer cannot sell or otherwise transfer this product, its components or materials made using this product or its components to a third party. Information about licenses for excluded uses is available from: E. I. du Pont de Nemours and Company; Attn: Associate Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881-9787 (voice), 1-302-695-1437 (fax),


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
A multicentric study to evaluate the use of relative retention times in targeted proteomics.
Vialas V, et al.
Journal of Proteomics, 152, 138-149 (2017)
Distinguishing between Leucine and Isoleucine by Integrated LC-MS Analysis Using an Orbitrap Fusion Mass Spectrometer.
Xiao Y, et al.
Analytical Chemistry, 88, 10757-10766 (2016)
Vital Vialas et al.
Journal of proteomics, 152, 138-149 (2016-12-19)
Despite the maturity reached by targeted proteomic strategies, reliable and standardized protocols are urgently needed to enhance reproducibility among different laboratories and analytical platforms, facilitating a more widespread use in biomedical research. To achieve this goal, the use of dimensionless...
Yongsheng Xiao et al.
Analytical chemistry, 88(21), 10757-10766 (2016-11-02)
Despite the great success of mass spectrometry (MS) for de novo protein sequencing, Leu and Ile have been generally considered to be indistinguishable by MS because their molecular masses are exactly the same. Positioning of incorrect Leu/Ile residues in variable...
Standards are critical in liquid chromatography/mass spectrometry (LC/MS) based proteomics to ensure optimal and consistent system performance before, during, and after sample analysis.
Read More

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