MS RT Calibration Mix has been used as a reference for iRT (relative retention times) calculations after LC-MS (liquid chromatography–mass spectrometry)/MS analysis.
[1] It has been used to check the reliability and specificity of ETD-HCD (electron transfer dissociation-high-energy collisional dissociation) MS
3 (mass spectrometry) method for leucine/isoleucine discrimination.
[2]The MS RT Calibration Mix is a LC-MS platform standard intended to test properties such as LC resolution, peptide elution profiles, and retention time prediction. The peptides in MSRT1 are designed to span the normal elution profile of complex proteomic samples. The peptides have been added in various amounts, based on electrospray response, to have relatively similar response and be readily observed.
MSRT1 is an injection-ready standard, and can serve for quick tests of LC-MS platform performance, comparison of LC gradients and columns, monitoring column and system changes. MSRT1 can be used to predict retention times of peptides across LC-MS platforms. MSRT1 is also applicable to multiple reaction monitoring / selected reaction monitoring (MRM / SRM) analysis.