MTOX1303
Canine MDR1 Knockout, Human MDR1 Knockin MDCKII Cells
canine kidney (cocker spaniel)
Synonym(s):
MDCKII hMDR1 knockin cells
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Product Name
Canine MDR1 Knockout, Human MDR1 Knockin MDCKII Cells,
biological source
canine kidney (cocker spaniel)
form
liquid
storage temp.
−196°C
Quality Level
Gene Information
human ... ABCB1(5243)
1 of 4
This Item | MTOX1304 | MTOX1301 | MTOX1300 |
|---|---|---|---|
| biological source canine kidney (cocker spaniel) | biological source canine kidney (cocker spaniel) | biological source canine kidney (cocker spaniel) | biological source canine |
| storage temp. −196°C | storage temp. −196°C | storage temp. −196°C | storage temp. −196°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| Gene Information human ... ABCB1(5243) | Gene Information human ... ABCG2(9429) | Gene Information - | Gene Information - |
| form liquid | form liquid | form liquid | form liquid |
Analysis Note
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.
Application
See technical bulletin for detailed protocols
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Features and Benefits
MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells.
The canine MDR1 (cP-gp) efflux transporter gene has been effectively disrupted in both alleles. There is no expression of the cP-gp. Human MDR1 (P-gp) has been transfected into the MDCKII cell line. Validation studies have shown efflux of standard MDR1 substrates.
Legal Information
This product is covered under a consumable purchase agreement for one-time use only. For more information:ADME/Tox Cell Lines LicenseMDCKII subclone was originally isolated by Daniel Louvard, Institut Curie, Paris France. [1][2][3][4]
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Stefan Gahbauer et al.
Proceedings of the National Academy of Sciences of the United States of America, 120(2), e2212931120-e2212931120 (2023-01-05)
The nonstructural protein 3 (NSP3) of the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) contains a conserved macrodomain enzyme (Mac1) that is critical for pathogenesis and lethality. While small-molecule inhibitors of Mac1 have great therapeutic potential, at the outset of the COVID-19
D Louvard
Proceedings of the National Academy of Sciences of the United States of America, 77(7), 4132-4136 (1980-07-01)
A dog kidney epithelial cell line (MDCK), grown in monolayer, displayed in vitro an asymmetric localization of surface proteins. Aminopeptidase [alpha-aminoacylpeptide hydrolase (microsomal), EC 3.4.11.2] was found only in the apical face whereas Na+, K+-ATPase (ATP phosphohydrolase, EC 3.6.1.3) was
Shehab Eid et al.
International journal of molecular sciences, 23(23) (2022-12-12)
Several strands of investigation have established that a reduction in the levels of the cellular prion protein (PrPC) is a promising avenue for the treatment of prion diseases. We recently described an indirect approach for reducing PrPC levels that targets
Deep Agnani et al.
PloS one, 6(10), e25086-e25086 (2011-10-27)
P-glycoprotein, a human multidrug resistance transporter, has been extensively studied due to its importance to human health and disease. In order to understand transport kinetics via P-gp, confluent cell monolayers overexpressing P-gp are widely used. The purpose of this study
G C Hansson et al.
The EMBO journal, 5(3), 483-489 (1986-03-01)
The glycosphingolipids (GSLs) of two sublines of Madin-Darby canine kidney (MDCK) cells, an epithelial cell line, were characterized by t.l.c., antibody overlay and mass spectrometry. The major characteristic which distinguishes the two MDCK cell strains is their trans-epithelial electrical resistance
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