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MilliporeSigma

P7173

Pyruvate Carboxylase from bovine liver

buffered aqueous glycerol solution, 5-25 units/mg protein (BCA)

Synonym(s):

Pyruvate:CO2 ligase (ADP-forming)

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10 UNITS

$987.00

$987.00

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About This Item

CAS Number:
9014-19-1
UNSPSC Code:
12352204
NACRES:
NA.26
EC Number:
6.4.1.1 (BRENDA, IUBMB)
MDL number:
MFCD00132143

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Product Name

Pyruvate Carboxylase from bovine liver, buffered aqueous glycerol solution, 5-25 units/mg protein (BCA)

form

buffered aqueous glycerol solution

specific activity

5-25 units/mg protein (BCA)

concentration

≥0.5 mg/mL

foreign activity

lactic dehydrogenase ≤0.5%

storage temp.

−20°C

Quality Level

200

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This Item
A5043L2254G1270
Pyruvate Carboxylase from bovine liver buffered aqueous glycerol solution, 5-25 units/mg protein (BCA)

Sigma-Aldrich

P7173

Pyruvate Carboxylase from bovine liver

Adenosine Deaminase from bovine spleen Type X, buffered aqueous glycerol solution, ≥130 units/mg protein

Sigma-Aldrich

A5043

Adenosine Deaminase from bovine spleen

Lipoprotein Lipase from bovine milk ammonium sulfate suspension, ≥2,000 units/mg protein (BCA)

Sigma-Aldrich

L2254

Lipoprotein Lipase from bovine milk

L-Glutamine Synthetase from Escherichia coli lyophilized powder, 400-2,000 units/mg protein

Sigma-Aldrich

G1270

L-Glutamine Synthetase from Escherichia coli

specific activity

5-25 units/mg protein (BCA)

specific activity

≥130 units/mg protein

specific activity

≥2,000 units/mg protein (BCA)

specific activity

400-2,000 units/mg protein

concentration

≥0.5 mg/mL

concentration

-

concentration

-

concentration

-

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

ammonium sulfate suspension

form

lyophilized powder

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

foreign activity

lactic dehydrogenase ≤0.5%

foreign activity

alkaline phosphatase ≤0.01%, guanase ≤0.01%, nucleoside phosphorylase ≤0.1%

foreign activity

-

foreign activity

ATPase <0.2%

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Pyruvate is critical for gluconeogenesis, lipogenesis, glyceroneogenesis, neurotransmitter biosynthesis and glucose-induced insulin, and is used to study these processes.
The enzyme from Sigma has been used as a positive control during the assay of pyruvate carboxylase activity in cell-free extracts of Corynebacterium glutamicum.[1]

Biochem/physiol Actions

Pyruvate carboxylase catalyzes the carboxylation of pyruvate to oxaloacetate. Pyruvate carboxylase is a mitochondrial protein that has a biotin prosthetic group that requiries magnesium or manganese and acetyl CoA[2].

Other Notes

One unit will convert 1.0 μmole of pyruvate and CO2 to oxalacetate per min at pH 7.8 at 30 °C.

Physical form

Solution in 50% glycerol containing 0.05 M Tris-HCl, pH 7.4, 2 mM magnesium acetate and 1 mM EDTA.

Preparation Note

Affinity purified

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000299813
0000299813
0000259278
0000259278
SLBF4710

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Philip Lee et al.
Hepatology (Baltimore, Md.), 57(2), 515-524 (2012-08-23)
The pathogenesis of type 2 diabetes is characterized by impaired insulin action and increased hepatic glucose production (HGP). Despite the importance of hepatic metabolic aberrations in diabetes development, there is currently no molecular probe that allows measurement of hepatic gluconeogenic
Eunsook S Jin et al.
Metabolism: clinical and experimental, 62(1), 152-162 (2012-09-18)
A three-day high-fat diet induces hepatic steatosis and hepatic insulin resistance in rats without altering fasting plasma glucose concentration or the rate of glucose production. However, as the nutrient profile available to the liver is substantially altered by a high-fat
Abdussalam Adina-Zada et al.
Biochemistry, 51(41), 8208-8217 (2012-09-19)
Mutation of Arg427 and Arg472 in Rhizobium etli pyruvate carboxylase to serine or lysine greatly increased the activation constant (K(a)) of acetyl CoA, with the increase being greater for the Arg472 mutants. These results indicate that while both these residues
H M White et al.
Journal of dairy science, 95(3), 1249-1256 (2012-03-01)
Expression of mRNA for pyruvate carboxylase (PC) is elevated at calving and during other physiological states when plasma NEFA concentrations are increased. The objective of this study was to determine the direct effects of fatty acids on expression of PC
Scott B Crown et al.
BMC systems biology, 6, 43-43 (2012-05-18)
¹³C-Metabolic flux analysis (¹³C-MFA) is a standard technique to probe cellular metabolism and elucidate in vivo metabolic fluxes. 13C-Tracer selection is an important step in conducting ¹³C-MFA, however, current methods are restricted to trial-and-error approaches, which commonly focus on an
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