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PROTSIL1
ProteoSilver™ Silver Stain Kit
Synonym(s):
Silver Staining Kit
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1 KIT
$335.00
$335.00
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About This Item
NACRES:
NA.32
UNSPSC Code:
41105322
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usage
kit sufficient for 25 mini-gels (10 × 10 cm)
technique(s)
protein staining: suitable
Quality Level
1 of 4
This Item | PROTSIL2 | S5692 | HT100A |
|---|---|---|---|
| technique(s) protein staining: suitable | technique(s) protein staining: suitable | technique(s) protein staining: suitable | technique(s) - |
| usage kit sufficient for 25 mini-gels (10 × 10 cm) | usage kit sufficient for 25 mini-gels (10 × 10 cm) | usage - | usage - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 500 |
Application
ProteoSilver™ Silver Stain Kit has been used for staining of the gels for visualizing proteins.[1]
Features and Benefits
- Premixed and preweighed solutions reduce time and cost of purchasing and preparing individual components
- Optimized protocols help establish conditions for best results
- High sensitivity and low background ensure very low abundance proteins can be detected and resolved from other proteins
- MALDI Compatible - Protein spots of interest can be further characterized by mass spectrometry
- Room Temperature Stability allows easy and convenient storage
General description
Conveniently packaged, extremely stable, and highly sensitive, ProteoSilver is an ideal product for any protein scientist. The kit contains prepared solutions of silver staining reagents along with detailed instructions to achieve optimal results. With a detection limit of 0.1 ng/mm2 of protein (BSA) and an extremely low background, ProteoSilver leads to superior detection of low abundance proteins.
Legal Information
ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
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Description
Pricing
signalword
Danger
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Muta. 2 - Repr. 1B - Skin Corr. 1A - Skin Sens. 1 - STOT SE 2 - STOT SE 3
target_organs
Eyes,Central nervous system, Respiratory system
Storage Class
3 - Flammable liquids
flash_point_f
132.8 °F - closed cup
flash_point_c
56 °C - closed cup
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Jean-Paul Lasserre et al.
Electrophoresis, 27(16), 3306-3321 (2006-07-22)
Study of the complexome - all the protein complexes of the cell - is essential for a better understanding and more global vision of cell function. Using two-dimensional blue native/SDS-PAGE (2-D BN/SDS-PAGE) technology, the cytosolic and membrane protein complexes of
Yufan Zhang et al.
Materials today. Bio, 15, 100317-100317 (2022-06-28)
Polymer dots (Pdots) have been applied to imaging lymph nodes (LNs) and lymphatic vessels (LVs) in living mice and rats. However, the mechanism of absorption, distribution, metabolism, and excretion of Pdots in LNs and LVs is still unclear. Therefore, the
Yong-Feng Han et al.
The Plant cell, 28(5), 1215-1229 (2016-04-27)
The mechanism by which MORPHEUS' MOLECULE1 (MOM1) contributes to transcriptional gene silencing has remained elusive since the gene was first identified and characterized. Here, we report that two Arabidopsis thaliana PIAS (PROTEIN INHIBITOR OF ACTIVATED STAT)-type SUMO E3 ligase-like proteins
Andrea Miccoli et al.
International journal of molecular sciences, 22(7) (2021-04-04)
In jawed vertebrates, adaptive immune responses are enabled by T cells. Two lineages were characterized based on their T cell receptor (TcR) heterodimers, namely αβ or γδ peptide chains, which display an Ig domain-type sequence that is somatically rearranged. γδ
Zongyu Gao et al.
Nature communications, 13(1), 716-716 (2022-02-09)
Mitogen-activated protein kinase (MAPK) cascades play an important role in innate immunity against various pathogens in plants and animals. However, we know very little about the importance of MAPK cascades in plant defense against viral pathogens. Here, we used a
Articles
Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
Protocols
Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
Resumen de la química del gel de poliacrilamida e instrucciones detalladas para preparar a mano geles de poliacrilamida con los kits de moldeo del gel bis-Tris TurboMix mPAGE®
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Quality release date
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What the expired date of this product
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This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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Can this silver stain kit stain proteins without cysteines?
1 answer-
This kit has not been tested for use in samples with specifically no cysteine. There is a modification in the protocol should the sample have few cysteine residues. While cysteine is important in the mechanism of silver staining, the link below indicates that it may be possible:
https://pubmed.ncbi.nlm.nih.gov/2426985/#:~:text=Proteins%20without%20cysteine%20stained%20negatively,they%20contained%20covalently%20attached%20lipids.See the link below to the product technical bulletin:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/156/682/protsil1bul.pdfHelpful?
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