R5636

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

buffered aqueous solution

• Synonym(s): Transfer RNA, tRNA
• CAS Number: 9014-25-9
• UNSPSC Code: 12352106
• NACRES: NA.55
• MDL number: MFCD00132200

Properties

• Product Name: Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae), buffered aqueous solution
• grade: Molecular Biology
• form: buffered aqueous solution
• concentration: ≥9 mg/mL
• foreign activity: DNase, Nickase, none detected
• shipped in: dry ice
• storage temp.: −20°C
• Quality Level: 200

Description

General description

Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation. tRNAs are approximately 80 nucleotides long RNA molecules with a cloverleaf shaped secondary structure and a tertiary L-shaped structure.[1]

Preparation Note

tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.

Application

Suitable for use as a carrier in nucleic acid purification and precipitation.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used:

• in carrier solution as a part of control used for reverse transcriptase – quantitative polymerase reaction (RT-qPCR) assay[2]
• as a component of prehybridization(4) and hybridization buffer in single-label in situ hybridization[3]
• as a carrier to enhance recovery of RNA from small numbers of cells[4]

Biochem/physiol Actions

Transfer RNAs (tRNAs) play an important role in translation. It is responsible for the addition of amino acids to ribosome for peptide chain formation. The tRNA contains an anticodon region for mRNA base pairing and two attachment regions: for amino acid binding and tRNA synthetase recognition. The ribosomal RNA facilitates the movement of tRNA along the mRNA.[1]