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R5636

Sigma-Aldrich

Ribonucleic acid, transfer from baker's yeast (S. cerevisiae)

buffered aqueous solution

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Synonym(s):
Transfer RNA, tRNA
CAS Number:
MDL number:
NACRES:
NA.55

grade

for molecular biology

Quality Level

form

buffered aqueous solution

concentration

≥9 mg/mL

foreign activity

DNase, Nickase, none detected

shipped in

dry ice

storage temp.

−20°C

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This Item
R8508R4752R6750
Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

dry ice

shipped in

-

shipped in

-

grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

concentration

≥9 mg/mL

concentration

9-11 mg/mL

concentration

-

concentration

-

General description

Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation. tRNAs are approximately 80 nucleotides long RNA molecules with a cloverleaf shaped secondary structure and a tertiary L-shaped structure.

Application

Suitable for use as a carrier in nucleic acid purification and precipitation.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used:
  • in carrier solution as a part of control used for reverse transcriptase – quantitative polymerase reaction (RT-qPCR) assay
  • as a component of prehybridization(4) and hybridization buffer in single-label in situ hybridization
  • as a carrier to enhance recovery of RNA from small numbers of cells

Biochem/physiol Actions

Transfer RNAs (tRNAs) play an important role in translation. It is responsible for the addition of amino acids to ribosome for peptide chain formation. The tRNA contains an anticodon region for mRNA base pairing and two attachment regions: for amino acid binding and tRNA synthetase recognition. The ribosomal RNA facilitates the movement of tRNA along the mRNA.

Components

tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.

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