This product should be stored in 2-8°C in powdered format. The solution can be stored at 2-8°C for several weeks or at -20°C for more extended periods.
S4014
Spectinomycin dihydrochloride pentahydrate
powder, suitable for cell culture and plant cell culture, BioReagent
Synonym(s):
Spectinomycin
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About This Item
Empirical Formula (Hill Notation):
C14H24N2O7 · 2HCl · 5H2O
CAS Number:
Molecular Weight:
495.35
NACRES:
NA.76
PubChem Substance ID:
UNSPSC Code:
12352207
EC Number:
244-554-3
MDL number:
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Product Name
Spectinomycin dihydrochloride pentahydrate, powder, BioReagent, suitable for cell culture
mode of action
protein synthesis | interferes
storage temp.
2-8°C
Quality Level
InChI key
DCHJOVNPPSBWHK-UXXUFHFZSA-N
InChI
1S/C14H24N2O7.2ClH.5H2O/c1-5-4-6(17)14(20)13(21-5)22-12-10(19)7(15-2)9(18)8(16-3)11(12)23-14;;;;;;;/h5,7-13,15-16,18-20H,4H2,1-3H3;2*1H;5*1H2/t5-,7-,8+,9+,10+,11-,12-,13+,14+;;;;;;;/m1......./s1
SMILES string
O.O.O.O.O.Cl.Cl.CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@]3(O)[C@@H](O[C@H](C)CC3=O)O[C@@H]2[C@H]1O
biological source
synthetic (inorganic)
product line
BioReagent
form
powder
potency
≥603 μg per mg
storage condition
(Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.)
technique(s)
cell culture | mammalian: suitable
cell culture | plant: suitable
impurities
endotoxin, tested
color
white to pale buff
solubility
H2O: soluble 50 mg/mL at
antibiotic activity spectrum
Gram-negative bacteria
Gram-positive bacteria
application(s)
agriculture
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This Item | S9007 | S2647 | 1618003 |
|---|---|---|---|
| antibiotic activity spectrum Gram-negative bacteria, Gram-positive bacteria | antibiotic activity spectrum Gram-negative bacteria, Gram-positive bacteria | antibiotic activity spectrum Gram-negative bacteria, Gram-positive bacteria | antibiotic activity spectrum - |
| mode of action protein synthesis | interferes | mode of action protein synthesis | interferes | mode of action protein synthesis | interferes | mode of action - |
| technique(s) cell culture | mammalian: suitable, cell culture | plant: suitable | technique(s) - | technique(s) - | technique(s) - |
| product line BioReagent | product line - | product line - | product line - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| application(s) agriculture | application(s) agriculture | application(s) - | application(s) pharmaceutical (small molecule) |
Application
Spectinomycin dihydrochloride pentahydrate has been used as a supplement in different types of media.[1][2]
Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.
Biochem/physiol Actions
Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.
Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).
Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.
Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).
Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.
Disclaimer
Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.
General description
Chemical structure: aminoglycoside
Spectinomycin has generally been used for the treatment of gonorrhea in males, and a study conducted shows that spectinomycin dihydrochloride pentahydrate provides effective treatment against gonorrhea as well.[3] Clinically, spectinomycin dihydrochloride pentahydrate is easier to use in injections as it shows higher solubility than spectinomycin.[4]
Other Notes
Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.
Preparation Note
Stock solutions should be prepared at 10 mg/mL in water and filter sterilized.
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Spectinomycin: minimum inhibitory concentrations for Neisseria gonorrhoeae.
I A Porter et al.
The British journal of venereal diseases, 50(4), 289-290 (1974-08-01)
Spectinomycin dihydrochloride in the treatment of gonorrhoea in males.
R R Willcox
The British journal of clinical practice, 29(2), 34-36 (1975-02-01)
Songul Gurel et al.
Methods in molecular biology (Clifton, N.J.), 847, 109-122 (2012-02-22)
Successful efforts describing in vitro culturing, regeneration, and transformation of grain sorghum were reported, using particle bombardment, as early as 1993, and with Agrobacterium tumefaciens in 2000. Reported transformation efficiencies via Agrobacterium routinely range from 1 to 2%. Recently, such
Aditya C Bandekar et al.
Current biology : CB, 30(20), 3961-3971 (2020-09-12)
Although the major events in prokaryotic cell cycle progression are likely to be coordinated with transcriptional and metabolic changes, these processes remain poorly characterized. Unlike many rapidly growing bacteria, DNA replication and cell division are temporally resolved in mycobacteria, making
Robert A Seaborne et al.
The Journal of physiology, 597(14), 3727-3749 (2019-05-17)
We have recently identified that a HECT domain E3 ubiquitin ligase, named UBR5, is altered epigenetically (via DNA methylation) after human skeletal muscle hypertrophy, where its gene expression is positively correlated with increasing lean leg mass after training and retraining.
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