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MilliporeSigma

T8657

Trichloroacetic acid

suitable for electrophoresis, suitable for fixing solution (for IEF and PAGE gels), ≥99%

Synonym(s):

TCA

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$131.00

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$222.00

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$278.80

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About This Item

Linear Formula:
Cl3CCOOH
CAS Number:
Molecular Weight:
163.39
UNSPSC Code:
41105319
NACRES:
NB.22
PubChem Substance ID:
EC Number:
200-927-2
Beilstein/REAXYS Number:
970119
MDL number:

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Product Name

Trichloroacetic acid, suitable for electrophoresis, suitable for fixing solution (for IEF and PAGE gels), ≥99%

InChI

1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)

SMILES string

OC(=O)C(Cl)(Cl)Cl

InChI key

YNJBWRMUSHSURL-UHFFFAOYSA-N

grade

electrophoresis grade

vapor density

<1 (vs air)

vapor pressure

1 mmHg ( 51 °C)

assay

≥99%

form

powder or flakes (or crystals)

technique(s)

electrophoresis: suitable

turbidity

≤3 NTU (1 g/ml)

refractive index

n20/D 1.62 (lit.)

bp

196 °C (lit.)

mp

54-58 °C (lit.)

solubility

water: 1 g/mL, clear, colorless

density

1.62 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤0.001%
nitrate (NO3-): ≤0.002%
phosphate (PO43-): ≤5 ppm
sulfate (SO42-): ≤0.02%

cation traces

Fe: ≤0.0005%
heavy metals (as Pb): ≤0.002%

suitability

suitable for fixing solution (for IEF and PAGE gels)

application(s)

cell analysis

storage temp.

2-8°C

Quality Level

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1 of 4

This Item
T48858.22342T0699
assay

≥99%

assay

≥99.0% (titration)

assay

≥98% (acidimetric)

assay

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder or flakes (or crystals)

form

powder or crystals

form

solid

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-30°C

storage temp.

-

solubility

water: 1 g/mL, clear, colorless

solubility

H2O: 1 g/10 mL

solubility

1300 g/L

solubility

-

vapor density

<1 (vs air)

vapor density

<1 (vs air)

vapor density

-

vapor density

-

Application

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.
Trichloroacetic acid has been used:
  • for hydrolysis of DNA isolated from the cell[1]
  • for precipitation of glutathione isolated from seminal plasma[2]
  • as a fixative in microscopy[3]

General description

Trichloroacetic acid (TCA) is produced during the chlorination of water comprising natural organic material.[4] It is a novel reagent used in clinical laboratories for deproteinization of body fluids.[5] In addition, TCA is also used in the treatment of genital warts[6] and acne scars.[7]

pictograms

CorrosionEnvironment

signalword

Danger

hcodes

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk

WGK 2

flash_point_f

>235.4 °F - closed cup

flash_point_c

> 113 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Certificates of Analysis (COA)

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Determination of total protein in spinal fluid with sulphosalicylic acid and trichloroacetic acid
Meulemans O
Clinica Chimica Acta; International Journal of Clinical Chemistry, 5(5), 757-761 (1960)
Teratogenic activity of trichloroacetic acid in the rat
Smith MK, et al.
Teratology, 40(5), 445-451 (1989)
Quantitation of DNA fragmentation with diphenylamine
Boraschi D and Maurizi G
Apoptosis-a laboratory manual of experimental methods, 153-161 (1998)
Atmospheric pressure pulsed plasma induces cell death in photosynthetic organs via intracellularly generated ROS
Seol Y, et al.
Scientific reports, 7(1), 589-589 (2017)
Focal treatment of acne scars with trichloroacetic acid: chemical reconstruction of skin scars method
Lee J, et al.
Dermatologic Surgery : Official Publication For American Society For Dermatologic Surgery [Et Al.], 28(11), 1017-1021 (2002)

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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