T8694

TrkB/Fc Chimera human

Name: TrkB/Fc Chimera human
Brand: SIGMA
Price: 994 USD
Availability: InStock

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

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About This Item

UNSPSC Code:

51111800

NACRES:

NA.32

MDL number:

MFCD03458837

Key Documents

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Product Name

TrkB/Fc Chimera human, >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

biological source

human

recombinant

expressed in NSO cells

assay

>90% (SDS-PAGE)

form

lyophilized powder

potency

0.1-0.4 μg/mL ED₅₀

mol wt

120-130 kDa by SDS-PAGE (reducing)
calculated mol wt 71 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

western blot: suitable

impurities

endotoxin, tested

UniProt accession no.

Q16620

storage temp.

−20°C

Quality Level

200

Gene Information

human ... NTRK2(4915)

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1 of 4

This Item	A0476	E0278	E0528
Sigma-Aldrich T8694 TrkB/Fc Chimera human	Sigma-Aldrich A0476 Activin Receptor-Like Kinase 1/Fc Chimera human	Sigma-Aldrich E0278 Ephrin-A3/Fc Chimera human	Sigma-Aldrich E0528 Ephrin-A5/Fc Chimera human
technique(s) western blot: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) -
biological source human	biological source human	biological source human	biological source human
assay >90% (SDS-PAGE)	assay >90% (SDS-PAGE)	assay >90% (SDS-PAGE)	assay >90% (SDS-PAGE)
recombinant expressed in NSO cells	recombinant expressed in NSO cells	recombinant expressed in NSO cells	recombinant expressed in NSO cells
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
mol wt 120-130 kDa by SDS-PAGE (reducing), calculated mol wt 71 kDa	mol wt 50-55 kDa by SDS-PAGE (reducing), calculated mol wt ~37.3 kDa	mol wt monomer calculated mol wt 47.7 kDa, ~90 kDa by SDS-PAGE (reducing)	mol wt monomer calculated mol wt 48.6 kDa, 50-55 kDa by SDS-PAGE (reducing)

Analysis Note

Inhibits BDNF-induced proliferation of TrkB transfected cells, BaF-TrkB-BD.

Application

TrkB/Fc Chimera human can be used in synaptoneurosome preparation and western blot analysis.[1] Intracerebroventricular administeration of TrkB/Fc chimera human blocks TrkB ligand signaling and was used to study the role of BDNF in ATN response augmentation.[2]

Biochem/physiol Actions

Binds specifically to mediate neurotrophins BDNF and NT-4/5.

Member of the Trk family tyrosine kinase receptors. Binds specifically to mediate neurotrophins BDNF and NT-4/5.

TrkB/Fc Chimera human consist of extracellular domain of human TrkB1 fused to histidine-tagged Fc region of human IgG1 by using a polypeptide linker. TrkB belongs to tyrosine kinase receptors family and facilitates the hippocampal development as well as regulating the synaptic plasticity that underlies long-term potentiation and learning. Brain derived neurotrophic factor (BDNF) and Neurotrophin 4 or 5 are preferred ligands for TrkB. Nevertheless it can also bind to NTF3/neurotrophin-3 that is less efficient in activating the receptor but regulates neuron survival through NTRK2.[3]

Other Notes

Extracellular domain of human TrkB fused to the C-terminal histidine-tagged Fc region of human IgG1.

Physical form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline.

Preparation Note

Reconstitute to 100 μg/mL in phosphate buffered saline.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Differential regulation of synaptic plasticity of the hippocampal and the hypothalamic inputs to the anterior thalamus.

Marian Tsanov et al.

Hippocampus, 21(1), 1-8 (2010-01-01)

The hippocampus projects to the anterior thalamic nuclei both directly and indirectly via the mammillary bodies, but little is known about the electrophysiological properties of these convergent pathways. Here we demonstrate, for the first time, the presence of long-term plasticity

Mesocortical BDNF signaling mediates antidepressive-like effects of lithium.

Di Liu et al.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 45(9), 1557-1566 (2020-05-20)

Lithium has been used to treat major depressive disorder, yet the neural circuit mechanisms underlying this therapeutic effect remain unknown. Here, we demonstrated that the ventral tegmental area (VTA) dopamine (DA) neurons that project to the medial prefrontal cortex (mPFC)

Formation of aversive memories associated with conditioned drug withdrawal requires BDNF expression in the amygdala in acute morphine-dependent rats.

Yun-yue Ju et al.

Acta pharmacologica Sinica, 36(12), 1437-1443 (2015-11-17)

Brain-derived neurotrophic factor (BDNF) plays an important role in learning and memory in multiple brain areas. In the present study, we investigated the roles of BDNF in aversive memories associated with conditioned drug withdrawal in acute morphine-dependent rats. Conditioned place

A de novo mutation affecting human TrkB associated with severe obesity and developmental delay.

Giles S H Yeo et al.

Nature neuroscience, 7(11), 1187-1189 (2004-10-21)

An 8-year-old male with a complex developmental syndrome and severe obesity was heterozygous for a de novo missense mutation resulting in a Y722C substitution in the neurotrophin receptor TrkB. This mutation markedly impaired receptor autophosphorylation and signaling to MAP kinase.

Accumbens brain-derived neurotrophic factor (BDNF) transmission inhibits cocaine seeking.

Ana-Clara Bobadilla et al.

Addiction biology, 24(5), 860-873 (2018-06-12)

Brain-derived neurotrophic factor (BDNF) regulates a variety of physiological processes, and several studies have explored the role of BDNF in addiction-related brain regions like the nucleus accumbens core (NAcore). We sought to understand the rapid effects of endogenous BDNF on

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