T8700

Sigma-Aldrich

Anti-Tyrosine Hydroxylase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution, sufficient for 10 blots

MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

usage

sufficient for 10 blots

species reactivity

mammals

application(s)

immunohistochemistry (frozen sections): 1:1,000
indirect immunofluorescence: 1:1,000
western blot: 1:1,000 using rat brain lysates of PC-12 cells stimulated by okadaic acid

conjugate

unconjugated

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TH(7054)
mouse ... Th(21823)
rat ... Th(25085)

General description

Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic, and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH4) and O2 as cosubstrates, and Fe2+ as a cofactor.

Immunogen

SDS-denatured rat tyrosine hydroxylase purified from pheochromocytoma.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Anti-tyrosine hydroxylase antibody produced in rabbit has been used in western blot analysis and immunohistochemistry.

Biochem/physiol Actions

Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson′s disease (PD).

Target description

Tyrosine hydroxylase (TH) is involved in the conversion of phenylalanine to dopamine. It catalyzes the initial, rate-limiting step of the catecholamine biosynthetic pathway.

Physical form

Solution in 100 μL in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Cathy Cailotto et al.
PloS one, 9(1), e87785-e87785 (2014-02-04)
Electrical stimulation of the vagus nerve suppresses intestinal inflammation and normalizes gut motility in a mouse model of postoperative ileus. The exact anatomical interaction between the vagus nerve and the intestinal immune system remains however a matter of debate. In...
Anne Grünewald et al.
Journal of neuroscience methods, 232, 143-149 (2014-06-01)
Respiratory chain (RC) deficiencies are found in primary mtDNA diseases. Focal RC defects are also associated with ageing and neurodegenerative disorders, e.g. in substantia nigra (SN) neurons from Parkinson's disease patients. In mitochondrial disease and ageing, mtDNA mutational loads vary...
Laura C Skoczylas et al.
Reproductive sciences (Thousand Oaks, Calif.), 20(4), 382-390 (2013-01-10)
The objective of this study was to define the regional differences in rat vaginal smooth muscle contractility and morphology. We evaluated circumferential segments from the proximal, middle, and distal rat vagina (n = 21) in vitro. Contractile responses to carbachol...
Hui-Ling Diao et al.
Frontiers in physiology, 8, 897-897 (2017-11-23)
The globus pallidus is a central nucleus in the basal ganglia motor control circuit. Morphological studies have revealed the expression of adenosine A2A receptors in the globus pallidus. To determine the modulation of adenosine A2A receptors on the activity of...
Anne Grünewald et al.
Annals of neurology, 79(3), 366-378 (2015-11-26)
To determine the extent of respiratory chain abnormalities and investigate the contribution of mtDNA to the loss of respiratory chain complexes (CI-IV) in the substantia nigra (SN) of idiopathic Parkinson disease (IPD) patients at the single-neuron level. Multiple-label immunofluorescence was...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service