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T8700

Sigma-Aldrich

Anti-Tyrosine Hydroxylase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution, sufficient for 10 blots

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Synonym(s):
Rabbit Anti-TYH
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

usage

sufficient for 10 blots

species reactivity

mammals

technique(s)

immunohistochemistry (frozen sections): 1:1,000
indirect immunofluorescence: 1:1,000
western blot: 1:1,000 using rat brain lysates of PC-12 cells stimulated by okadaic acid

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TH(7054)
mouse ... Th(21823)
rat ... Th(25085)

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T1299SAB4502964SAB2701683
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

ascites fluid

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

TH-2, monoclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous glycerol solution

form

-

form

buffered aqueous solution

form

buffered aqueous solution

species reactivity

mammals

species reactivity

human, monkey, guinea pig, rat, sheep, bovine, rabbit

species reactivity

mouse, rat, human

species reactivity

zebrafish

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General description

Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic, and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH4) and O2 as cosubstrates, and Fe2+ as a cofactor.

Immunogen

SDS-denatured rat tyrosine hydroxylase purified from pheochromocytoma.

Application

Anti-tyrosine hydroxylase antibody produced in rabbit has been used in western blot analysis and immunohistochemistry.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Biochem/physiol Actions

Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson′s disease (PD).

Target description

Tyrosine hydroxylase (TH) is involved in the conversion of phenylalanine to dopamine. It catalyzes the initial, rate-limiting step of the catecholamine biosynthetic pathway.

Physical form

Solution in 100 μL in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Maheep Bhatnagar et al.
PloS one, 12(5), e0177254-e0177254 (2017-05-17)
Parkinson's disease remains as one of the most common debilitating neurodegenerative disorders. With the hopes of finding agents that can cure or reduce the pace of progression of the disease, we studied two traditional medicinal plants: Centella asiatica and Withania
Z Jallah et al.
BJOG : an international journal of obstetrics and gynaecology, 123(7), 1076-1085 (2015-08-25)
To evaluate the impact of prolapse meshes on vaginal smooth muscle structure (VaSM) and function, and to evaluate these outcomes in the context of the mechanical and textile properties of the mesh. Three months following the implantation of three polypropylene
Chi-Lin Li et al.
Nutrients, 11(7) (2019-07-25)
Schisandra chinensis (Turcz.) Baill. (S. chinensis) is a well-known botanical medicine and nutritional supplement that has been shown to have potential effects on neurodegeneration. To investigate the potential neuroprotective effect of S. chinensis fruit extract, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was used to
Yanqin Wang et al.
PPAR research, 2017, 4089214-4089214 (2017-03-31)
The aim of the present study was to gain insight into the neuroprotection effects and mechanism of thiazolidinedione pioglitazone in both in vitro and in vivo MPP+/MPTP induced PD models. In vivo experimental results showed that oral treatment of pioglitazone
Jorge Mendoza et al.
Journal of neurochemistry, 112(6), 1489-1499 (2010-01-14)
Under special restricted feeding conditions the mammalian circadian clock, contained in the hypothalamic suprachiasmatic nucleus (SCN), can be entrained by food. During food restriction, hungry animals are very motivated to obtain food. This motivational state could be a key component

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