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Milk Macrophage Function in Bovine Leukemia Virus-Infected Dairy Cows.

Frontiers in veterinary science (2021-07-06)
Ewerton de Souza Lima, Maiara Garcia Blagitz, Camila Freitas Batista, Alexandre José Alves, Artur Cezar de Carvalho Fernandes, Eduardo Milton Ramos Sanchez, Hugo Frias Torres, Soraia Araújo Diniz, Marcos Xavier Silva, Alice Maria Melville Paiva Della Libera, Fernando Nogueira de Souza
ABSTRACT

The implications of bovine leukemia virus (BLV) on innate and adaptive immune responses have been widely investigated; however, the effects of BLV on mammary gland immunity require further investigation. The present study investigated the viability, phagocytic capacity, and intracellular production of reactive oxygen and nitrogen species (RONS) by macrophages in milk samples from dairy cows naturally infected with BLV with or without persistent lymphocytosis (PL). No effect of BLV infection in the overall number of macrophages per milliliter and in the percentage of viable macrophages among overall milk viable cells was found. Furthermore, BLV-infected dairy cows had a higher frequency of viable milk macrophages, while healthy animals had a tendency toward a higher percentage of apoptotic milk macrophages. The percentage of milk macrophages that phagocytosed Staphylococcus aureus in seronegative animals was higher than that in BLV-infected dairy cows. No effect of BLV infection on the intracellular RONS production and the intensity of phagocytosis by milk macrophages was observed. Thus, this study provides new insights into the implications of BLV infections in the bovine mammary gland.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
RPMI-1640 Medium, Dutch Modification, with sodium bicarbonate and 20mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
2′,7′-Dichlorofluorescin diacetate, ≥97%