R7638

RPMI-1640 Medium

Dutch Modification, with sodium bicarbonate and HEPES, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): Roswell Park Memorial Institute 1640 medium
• UNSPSC Code: 12352207
• NACRES: NA.75
• MDL number: MFCD00217820

Properties

• Product Name: RPMI-1640 Medium, Dutch Modification, with sodium bicarbonate and 20mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• pH: >7.2
• components: sodium pyruvate: no; HEPES: 20 mM; L-glutamine: no; NaHCO₃: yes; phenol red: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used

• to obtain spleen and lymph nodes from mice[1]
• for the resuspension of Staphylococcus aureus strains in the phagocytosis assay[2]
• in the isolation of hepatitis B surface antigen-specific B-cell clones

Preparation Note

Supplement with 0.3 g/L L-glutamine.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable