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Effect of culture conditions on microorganism identification by matrix-assisted laser desorption ionization mass spectrometry.

Applied and environmental microbiology (2005-01-11)
Nancy Valentine, Sharon Wunschel, David Wunschel, Catherine Petersen, Karen Wahl
ABSTRACT

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has been used to identify bacteria based upon protein signatures. This research shows that while some different proteins are produced by vegetative bacteria when they are cultured in different growth media, positive identification with MALDI-TOF MS is still possible with the protocol established at the Pacific Northwest National Laboratory (K. H. Jarman, S. T. Cebula, A. J. Saenz, C. E. Petersen, N. B. Valentine, M. T. Kingsley, and K. L. Wahl, Anal. Chem. 72:1217-1223, 2000). A core set of small proteins remain constant under at least four different culture media conditions and blood agar plates, including minimal medium M9, rich media, tryptic soy broth (TSB) or Luria-Bertani (LB) broth, and blood agar plates, such that analysis of the intact cells by matrix-assisted laser desorption/ionization mass spectrometry allows for consistent identification.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
M9, Minimal Salts, 5X, powder, minimal microbial growth medium
Millipore
M9 Broth, suitable for microbiology, NutriSelect® Basic

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