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Ethyl acetate as a substitute for diethyl ether in the formalin-ether sedimentation technique.

Journal of clinical microbiology (1979-12-01)
K H Young, S L Bullock, D M Melvin, C L Spruill
PMID574877
ABSTRACT

Ethyl acetate appears to be a satisfactory subsitute solvent for diethyl ether in the Formalin-ether sedimentation technique. In comparative studies, concentration of organisms with ethyl acetate was equal to or greater than that with diethyl ether. No distortion or alteration of morphology was observed with eigher solvent, and preparations were comparable in appearance and ease of examination. In addition, ethyl acetate is less flammable and less hazardous to use than diethyl ether.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ethyl acetate, anhydrous, 99.8%
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Diethyl ether, contains 1 ppm BHT as inhibitor, anhydrous, ≥99.7%
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Diethyl ether, suitable for HPLC, ≥99.9%, inhibitor-free
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Diethyl ether, contains BHT as inhibitor, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%
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Diethyl ether, puriss., contains ~5 mg/L 2,6-di-tert.-butyl-4-methylphenol as stabilizer, meets analytical specification of Ph. Eur., BP, ≥99.5% (GC)
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Ethyl acetate, suitable for HPLC, ≥99.8%
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Ethyl acetate, ReagentPlus®, ≥99.8%
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Ethyl acetate, ACS reagent, ≥99.5%
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Ethyl acetate, biotech. grade, ≥99.8%
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Ethyl acetate, ACS reagent, ≥99.5%