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Production of P-aminobenzoic acid by metabolically engineered escherichia coli.

Bioscience, biotechnology, and biochemistry (2014-07-19)
Daisuke Koma, Hayato Yamanaka, Kunihiko Moriyoshi, Kiyofumi Sakai, Takaya Masuda, Yoshihiro Sato, Kozo Toida, Takashi Ohmoto

The production of chemical compounds from renewable resources is an important issue in building a sustainable society. In this study, Escherichia coli was metabolically engineered by introducing T7lac promoter-controlled aroF(fbr), pabA, pabB, and pabC genes into the chromosome to overproduce para-aminobenzoic acid (PABA) from glucose. Elevating the copy number of chromosomal PT7lac-pabA-pabB distinctly increased the PABA titer, indicating that elevation of 4-amino-4-deoxychorismic acid synthesis is a significant factor in PABA production. The introduction of a counterpart derived from Corynebacterium efficiens, pabAB (ce), encoding a fused PabA and PabB protein, resulted in a considerable increase in the PABA titer. The introduction of more than two copies of PT7lac-pabAB (ce-mod), a codon-optimized pabAB (ce), into the chromosome of a strain that simultaneously overexpressed aroF(fbr) and pabC resulted in 5.1 mM PABA from 55.6 mM glucose (yield 9.2%). The generated strain produced 35 mM (4.8 g L(-1)) PABA from 167 mM glucose (yield 21.0%) in fed-batch culture.

Product Number
Product Description

4-Aminobenzoic acid, analytical standard
4-Aminobenzoic acid, European Pharmacopoeia (EP) Reference Standard
4-Aminobenzoic acid, purified by sublimation, ≥99%
4-Aminobenzoic acid, ReagentPlus®, 99%
4-Aminobenzoic acid, ReagentPlus®, ≥99%