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Human CD141+ dendritic cells induce CD4+ T cells to produce type 2 cytokines.

Journal of immunology (Baltimore, Md. : 1950) (2014-09-24)
Chun I Yu, Christian Becker, Patrick Metang, Florentina Marches, Yuanyuan Wang, Hori Toshiyuki, Jacques Banchereau, Miriam Merad, A Karolina Palucka
ABSTRACT

Dendritic cells (DCs) play the central role in the priming of naive T cells and the differentiation of unique effector T cells. In this study, using lung tissues and blood from both humans and humanized mice, we analyzed the response of human CD1c(+) and CD141(+) DC subsets to live-attenuated influenza virus. Specifically, we analyzed the type of CD4(+) T cell immunity elicited by live-attenuated influenza virus-exposed DCs. Both DC subsets induce proliferation of allogeneic naive CD4(+) T cells with the capacity to secrete IFN-γ. However, CD141(+) DCs are uniquely able to induce the differentiation of IL-4- and IL-13-producing CD4(+) T cells. CD141(+) DCs induce IL-4- and IL-13-secreting CD4(+) T cells through OX40 ligand. Thus, CD141(+) DCs demonstrate remarkable plasticity in guiding adaptive immune responses.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
Brefeldin A, from Penicillium brefeldianum, ≥99% (HPLC and TLC)
Sigma-Aldrich
Brefeldin A, ≥99% (HPLC and TLC), BioXtra, for molecular biology
Sigma-Aldrich
Brefeldin A, from Penicillium brefeldianum, Ready Made Solution, 10 mg/mL in DMSO
Sigma-Aldrich
TSLP human, recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC)