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Nanoplatforms attached Schiff bases by condensation method; Investigation of glucose oxidase enzyme as biocatalysts.

Artificial cells, nanomedicine, and biotechnology (2013-11-22)
Seda Kayhan, Nurşen Sarı, Dilek Nartop
ABSTRACT

We report a easy approach for the immobilization of glucose oxidase enzyme (GOx) on nanoparticles. Nanoparticles-modified polystyrene-ANH2 (PSA) with some salicylaldehyde derivatives were synthesized by means of condensation and investigated the enzymatic properties of GOx immobilized on there. Modified polystyrenes were characterized using IR spectra, gel permeation chromatography, and scanning electron microscopy. All the characteristics of the immobilized glucose oxidase (PSA-SalH)-GOx, (PSA-SalCH3)-GOx, and (PSA)-GOx enzyme showed except one of them. Immobilized GOx on to (PSA) showed two optimum pH, other immobilized polymers were one optimum.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
N,N-Dimethylformamide, anhydrous, 99.8%
Sigma-Aldrich
Phenol solution, BioReagent, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, for molecular biology
Sigma-Aldrich
N,N-Dimethylformamide, for molecular biology, ≥99%
Sigma-Aldrich
Phenol, puriss. p.a., ACS reagent, reag. Ph. Eur., 99.0-100.5%
Sigma-Aldrich
Phenol, for molecular biology
Sigma-Aldrich
Salicylaldehyde, reagent grade, 98%
Sigma-Aldrich
Liquified Phenol, ≥89.0%
Sigma-Aldrich
Phenol, BioXtra, ≥99.5% (GC)
Sigma-Aldrich
Phenol solution, Saturated with 0.1 M citrate buffer, pH 4.3 ± 0.2, BioReagent, for molecular biology
Sigma-Aldrich
Phenol, ≥99%
Sigma-Aldrich
Phenol, contains hypophosphorous as stabilizer, loose crystals, ACS reagent, ≥99.0%
Sigma-Aldrich
Phenol, unstabilized, ReagentPlus®, ≥99%
Sigma-Aldrich
Phenol, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (GC)
Sigma-Aldrich
Salicylaldehyde, redist., ≥99.0% (GC)
Sigma-Aldrich
Phenol, BioUltra, for molecular biology, TE-saturated, ~73% (T)
Sigma-Aldrich
Phenol, puriss., ≥99.5% (GC), meets analytical specification of Ph. Eur., BP, USP, crystalline (detached)
Sigma-Aldrich
Phenol, ≥96.0% (calc. on dry substance, T)
Sigma-Aldrich
N,N-Dimethylformamide, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Salicylaldehyde, ≥98%, FG
Sigma-Aldrich
Phenol, unstabilized, purified by redistillation, ≥99%
Sigma-Aldrich
Phenol, natural, 97%, FG
Sigma-Aldrich
2-Hydroxy-5-methylbenzaldehyde, 98%
Sigma-Aldrich
N,N-Dimethylformamide, ACS reagent, ≥99.8%