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D4551

N,N-Dimethylformamide

Molecular Biology, ≥99%

N,N-Dimethylformamide
1 of 1 reviewers took part in a promotion

Synonym(s):

DMF, NSC 5356

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About This Item

Linear Formula:
HCON(CH3)2
CAS Number:
68-12-2
Molecular Weight:
73.09
UNSPSC Code:
12352111
NACRES:
NA.52
PubChem Substance ID:
24893883
EC Number:
200-679-5
Beilstein/REAXYS Number:
605365
MDL number:
MFCD00003284
Grade:
Molecular Biology
Assay:
≥99%
Bp:
153 °C (lit.)
Vapor pressure:
2.7 mmHg ( 20 °C)

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Product Name

N,N-Dimethylformamide, Molecular Biology, ≥99%

InChI key

ZMXDDKWLCZADIW-UHFFFAOYSA-N

InChI

1S/C3H7NO/c1-4(2)3-5/h3H,1-2H3

SMILES string

[H]C(=O)N(C)C

grade

Molecular Biology

vapor density

2.5 (vs air)

vapor pressure

2.7 mmHg ( 20 °C)

assay

≥99%

form

liquid

autoignition temp.

833 °F

expl. lim.

15.2 %

impurities

≤0.5% water
water

refractive index

n20/D 1.430 (lit.)

bp

153 °C (lit.)

mp

−61 °C (lit.)

density

0.944 g/mL (lit.)

suitability

suitable for solvent for chromogenic substrates (X-Gal, etc.) used in molecular biology applications.

storage temp.

room temp

Quality Level

200

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1 of 4

This Item
22705633120437573
N,N-Dimethylformamide Molecular Biology, ≥99%

Sigma-Aldrich

D4551

N,N-Dimethylformamide

N,N-Dimethylformamide anhydrous, 99.8%

Sigma-Aldrich

227056

N,N-Dimethylformamide

N,N-Dimethylformamide puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.8% (GC)

Sigma-Aldrich

33120

N,N-Dimethylformamide

N,N-Dimethylformamide ACS reagent, ≥99.8%

Sigma-Aldrich

437573

N,N-Dimethylformamide

grade

for molecular biology

grade

anhydrous

grade

ACS reagent, puriss. p.a.

grade

ACS reagent

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

assay

≥99%

assay

99.8%

assay

≥99.8% (GC)

assay

≥99.8%

form

liquid

form

liquid

form

liquid

form

liquid

autoignition temp.

833 °F

autoignition temp.

833 °F

autoignition temp.

833 °F

autoignition temp.

833 °F

density

0.944 g/mL (lit.)

density

0.944 g/mL (lit.)

density

0.948-0.950 g/mL at 20 °C, 0.944 g/mL (lit.)

density

0.944 g/mL (lit.)

Application

N,N-Dimethylformamide was used to solubilize MTT crystals in cell viability assays.[1] It was also used in feruloyl esterase activity assay in molds exhibiting high activity of the enzyme.[2]
Solvent for many hydrophobic organic compounds.

General description

N, N-Dimethylformamide (DMF) is a solvent with a low evaporation rate, useful for preparing solutions with a variety of hydrophobic organic compounds used in molecular biology applications.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

Storage Class

3 - Flammable liquids

wgk

WGK 2

flash_point_f

135.5 °F - closed cup

flash_point_c

57.5 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS5646
BCCM9817
BCCL3950
SHBR3319
BCCM0887

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Lauren Friedman et al.
Anti-cancer drugs, 20(6), 444-449 (2009-04-23)
Curcumin, a yellow pigment and the active component of turmeric, has been shown to protect against carcinogenesis and prevent tumor development in several types of cancer. However, its low bioavailability and potency prevent it from being effective in most chemotherapeutic
M Kanauchi et al.
Journal of food science, 73(6), C458-C463 (2009-02-26)
For food industry production processes and other uses, a mold that produces high levels of feruloyl esterase was obtained from laboratory mold collections and other sources. It was Aspergillus awanmori G-2 that produces high levels of feruloyl esterase. The feruloyl
Ruiqin Wu et al.
Food microbiology, 84, 103236-103236 (2019-08-20)
Hepatitis A virus (HAV) continues to be the leading cause of viral hepatitis. HAV outbreaks have been linked to the consumption of milk, but methods for HAV detection in milk are very limited. We developed a method to concentrate HAV
Takaki Yamauchi et al.
Plant physiology, 169(1), 180-193 (2015-06-04)
In rice (Oryza sativa) roots, lysigenous aerenchyma, which is created by programmed cell death and lysis of cortical cells, is constitutively formed under aerobic conditions, and its formation is further induced under oxygen-deficient conditions. Ethylene is involved in the induction
Jixian Xu et al.
Nature communications, 6, 7081-7081 (2015-05-09)
Solution-processed planar perovskite devices are highly desirable in a wide variety of optoelectronic applications; however, they are prone to hysteresis and current instabilities. Here we report the first perovskite-PCBM hybrid solid with significantly reduced hysteresis and recombination loss achieved in
View All Related Papers

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Product Information Sheet - D4551

Product Information Sheet

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Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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Reviews

1 of 1 reviewers took part in a promotion

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    • Review 1
    • Vote 1
    5 out of 5 stars.

    Worked Well.

    Bought to create an X-gal solution for blue/white screening. Worked very well. X-Gal dissolved almost instantly.

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