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D4551

Sigma-Aldrich

N,N-Dimethylformamide

for molecular biology, ≥99%

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Synonym(s):
DMF, NSC 5356
Linear Formula:
HCON(CH3)2
CAS Number:
Molecular Weight:
73.09
Beilstein:
605365
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.52

grade

for molecular biology

Quality Level

vapor density

2.5 (vs air)

vapor pressure

2.7 mmHg ( 20 °C)

Assay

≥99%

form

liquid

autoignition temp.

833 °F

expl. lim.

15.2 %

impurities

≤0.5% water
water

refractive index

n20/D 1.430 (lit.)

bp

153 °C (lit.)

mp

−61 °C (lit.)

density

0.944 g/mL (lit.)

suitability

suitable for solvent for chromogenic substrates (X-Gal, etc.) used in molecular biology applications.

storage temp.

room temp

SMILES string

[H]C(=O)N(C)C

InChI

1S/C3H7NO/c1-4(2)3-5/h3H,1-2H3

InChI key

ZMXDDKWLCZADIW-UHFFFAOYSA-N

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This Item
494488319937437573
N,N-Dimethylformamide for molecular biology, ≥99%

Sigma-Aldrich

D4551

N,N-Dimethylformamide

-
N,N-Dimethylformamide biotech. grade, ≥99.9%

Sigma-Aldrich

494488

N,N-Dimethylformamide

-
N,N-Dimethylformamide ACS reagent, ≥99.8%

Sigma-Aldrich

319937

N,N-Dimethylformamide

Essential Grade
N,N-Dimethylformamide ACS reagent, ≥99.8%

Sigma-Aldrich

437573

N,N-Dimethylformamide

-
vapor density

2.5 (vs air)

vapor density

2.5 (vs air)

vapor density

2.5 (vs air)

vapor density

2.5 (vs air)

vapor pressure

2.7 mmHg ( 20 °C)

vapor pressure

2.7 mmHg ( 20 °C)

vapor pressure

2.7 mmHg ( 20 °C)

vapor pressure

2.7 mmHg ( 20 °C)

assay

≥99%

assay

≥99.9%

assay

≥99.8%

assay

≥99.8%

form

liquid

form

liquid

form

liquid

form

liquid

autoignition temp.

833 °F

autoignition temp.

833 °F

autoignition temp.

833 °F

autoignition temp.

833 °F

General description

N, N-Dimethylformamide (DMF) is a solvent with a low evaporation rate, useful for preparing solutions with a variety of hydrophobic organic compounds used in molecular biology applications.

Application

N,N-Dimethylformamide was used to solubilize MTT crystals in cell viability assays. It was also used in feruloyl esterase activity assay in molds exhibiting high activity of the enzyme.
Solvent for many hydrophobic organic compounds.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

135.5 °F - closed cup

Flash Point(C)

57.5 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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