P4682

Phenol solution

Saturated with 0.01 M citrate buffer, pH 4.3 ± 0.2, BioReagent, Molecular Biology

• Synonym(s): nucleic acid extraction
• Empirical Formula (Hill Notation): C₆H₆O
• CAS Number: 108-95-2
• Molecular Weight: 94.11
• Beilstein/REAXYS Number: 6428463
• MDL number: MFCD00002143
• UNSPSC Code: 12352104
• PubChem Substance ID: 57654523
• NACRES: NA.52
• Grade: Molecular Biology
• Technique(s): RNA purification: suitable

Properties

• grade: Molecular Biology
• Quality Level: 200
• product line: BioReagent
• form: liquid
• technique(s): RNA purification: suitable
• pH: 4.1-4.5 (25 °C)
• Storage temp.: 2-8°C
• SMILES string: Oc1ccccc1
• InChI: 1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
• InChI key: ISWSIDIOOBJBQZ-UHFFFAOYSA-N
• Gene Information: human ... GABRA1(2554)

Description

General description

Phenol solution is a clear liquid saturated with citrate buffer. Phenols are organic compounds that feature a hydroxyl group directly bonded to an aromatic carbocyclic ring. The common name for monohydroxybenzene is phenol.[1]

Application

Phenol solution has been used:

• for the extraction of RNA by standard phenol-chloroform protocols[2]
• in cell wall monosaccharide analysis to treat epidermal strips from the abaxial side of Commelina communisin leaves[3]
• as a component of lysis-denaturation buffer for RNA isolation[4]

Biochem/physiol Actions

Phenol solution can be used for a wide variety of molecular biology applications, including nucleic acid extractions using standard phenol-chloroform protocols. This organic solvent also serves as a potential protein denaturant. Phenol-chloroform extraction is a broadly used method for the extraction of nucleic acids. Despite phenol′s incomplete inhibition of RNase activity, it possesses characteristics that make it useful for isolating nucleic acids when combined with chloroform and alcohol.[5]

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H301 + H311 + H331,H314,H341,H373,H411
• pcodes: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• target_organs: Nervous system,Kidney,Liver,Skin
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable