Effect of Chaenomeles sinensis Extract on Choline Acetyltransferase Activity and Trimethyltin-Induced Learning and Memory Impairment in Mice.

Effect of Chaenomeles sinensis Extract on Choline Acetyltransferase Activity and Trimethyltin-Induced Learning and Memory Impairment in Mice.

Chemical & pharmaceutical bulletin (2015-12-04)

Yoon Kyung Kwon, Soo Jung Choi, Cho Rong Kim, Jae Kyeom Kim, Hye Kyung Kim, Jong Hun Choi, Sang-Wook Song, Chang-Ju Kim, Gwi Gun Park, Cheung-Seog Park, Dong-Hoon Shin

PMID26633030

ABSTRACT

The aim of this study was to search for a novel choline acetyltransferase (ChAT) activator from plants traditionally grown in Korea. An ethanol extract from Chaenomeles sinensis Koehne showed the highest ChAT-activating effect in vitro in an assay that used human neuroblastoma cells and [(14)C]acetyl-CoA. The active compound was speculated to be stearic acid methyl ester (SAME). In an in vivo experiment, C. sinensis extract and SAME improved trimethyltin (TMT)-induced deficits in learning and memory in mice as assessed by a Y-maze behavioral test and a passive avoidance test. The C. sinensis extract might attenuate the TMT-induced brain disorder. This study suggests that SAME from C. sinensis might be useful in the treatment of Alzheimer's disease.

MATERIALS

Product Number

Brand

Product Description

T25402

Sigma-Aldrich

Sodium tetraphenylborate, ACS reagent, ≥99.5%

648566

Sigma-Aldrich

Toluene, suitable for HPLC, ≥99.9%

Sigma-Aldrich

Ethylenediaminetetraacetic acid, purified grade, ≥98.5%, powder

C7527

Sigma-Aldrich

Choline chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%

E6758

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Ethylenediaminetetraacetic acid, anhydrous, crystalline, BioReagent, suitable for cell culture

EDS

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Ethylenediaminetetraacetic acid, BioUltra, anhydrous, ≥99% (titration)

431788

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Ethylenediaminetetraacetic acid, 99.995% trace metals basis

26978

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Choline chloride, BioUltra, ≥99.0% (AT)

72020

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Sodium tetraphenylborate, puriss. p.a., ACS reagent, ≥99.5% (NT)

244511

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Toluene, anhydrous, 99.8%

E8008

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Ethylenediaminetetraacetic acid solution, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

30-3304

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Toluene, JIS 1000, suitable for residue analysis, ≥99.8%

30-3280

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Toluene, JIS special grade, ≥99.5%

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Toluene, SAJ first grade, ≥99.0%

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Ethylenediaminetetraacetic acid, SAJ special grade, ≥99.0%

30-3302

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Toluene, JIS 300, suitable for residue analysis, ≥99.8%

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Choline chloride, ≥99%

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Ethanol, ≥99.5%, SAJ super special grade

09-0850

Sigma-Aldrich

Ethanol, ≥99.5%, suitable for absorption spectrum analysis

09-0851

Sigma-Aldrich

Ethanol, ≥99.5%, suitable for HPLC

09-0853

Sigma-Aldrich

Ethanol, ≥99.5%, suitable for fluorescence

09-0852

Sigma-Aldrich

Ethanol, ≥99.5%

09-0830

Sigma-Aldrich

Ethanol, JIS 300, ≥99.5%, suitable for residue analysis

09-0832

Sigma-Aldrich

Ethanol, JIS 1000, ≥99.5%, suitable for residue analysis

459836

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Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%

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Sigma-Aldrich

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