If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
E6758
Ethylenediaminetetraacetic acid
anhydrous, crystalline, BioReagent, suitable for cell culture
Synonym(s):
(Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid
About This Item
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grade
anhydrous
product line
BioReagent
assay
≥98.5%
form
crystalline
reaction suitability
reagent type: chelator
technique(s)
cell culture | mammalian: suitable
pH
2.5 (23 °C, 10 g/L)
mp
250 °C (dec.) (lit.)
solubility
3 M NaOH: 100 mg/mL
density
1.46 g/cm3 at 20 °C
cation traces
C: 40.7-41.5%
N: 9.3-9.8%
SMILES string
OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)
InChI key
KCXVZYZYPLLWCC-UHFFFAOYSA-N
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This Item | EDS | ED | E9884 |
|---|---|---|---|
| grade anhydrous | grade anhydrous | grade purified grade | grade ACS reagent |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) - |
| assay ≥98.5% | assay ≥99% (titration) | assay ≥98.5% | assay 99.4-100.6% |
| product line BioReagent | product line BioUltra | product line - | product line - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| mp 250 °C (dec.) (lit.) | mp 250 °C (dec.) (lit.) | mp 250 °C (dec.) (lit.) | mp 250 °C (dec.) (lit.) |
Application
- as a component of peptide dilution buffer for intracellular BH3 (Bcl-2 homology (BH) domain) profiling and validation[1]
- as a medium component in the hepatic stellate cell (HSCs) culture[2]
- as a component of homogenization buffer for protein extraction from eye tissues[3]
- for stopping benzonase reaction in lysis buffer[4]
Biochem/physiol Actions
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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