LC/MS/MS Analysis of Zopiclone and Metabolites on Ascentis® Express Phenyl-Hexyl
Materials
related product
Zopiclone solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Zopiclone-N-oxide solution
100 μg/mL (Methanol with 1% 1 M HCl), ampule of 1 mL, certified reference material, Cerilliant®N-Desmethylzopiclone solution
100 μg/mL in acetonitrile, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express Phenyl-Hexyl 10 cm x 2.1 mm, 2.7 μm particles (53336-U)
mobile phase
[A] 13 mM ammonium acetate in water, no pH adjustment; [B] 13 mM ammonium acetate in methanol; (52:48, A:B)
flow rate
0.4 mL/min
pressure
7850 psi (541 bar)
column temp.
35 °C
detector
MS, ESI(+), MRM
injection
1 μL
sample
250 ng/mL each in 13 mM ammonium acetate in water, no pH adjustment; [B] 13 mM ammonium acetate in methanol; (50:50 A:B)
Description
Analysis Note
Zopiclone is a non-benzodiazepine hypnotic sold under the trade names Imovane® and Zimovane and is prescribed for the treatment of insomnia. The Ascentis Express phenyl-Hexyl column gave the best separation of zopiclone and its metabolites of the column chemistries tested. Ultra high purity LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Other Notes
MRM Transitions
1 : 375.11 > 111.92 N-Desmethylzopiclone
2 : 375.11 > 217.00 N-Desmethylzopiclone
3 : 375.11 > 244.98 N-Desmethylzopiclone
4 : 389.08 > 111.92 Zopiclone
5 : 389.08 > 217.00 Zopiclone
6 : 389.08 > 244.97 Zopiclone
7 : 405.13 > 58.01 Zopiclone-N-oxide
8 : 405.13 > 143.29 Zopiclone-N-oxide
9 : 405.13 > 245.03 Zopiclone-N-oxide
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Imovane is a registered trademark of Sanofi-Aventis