The Jurkat E6.1 cell line is a banked product of ECACC and is tested sterility, viability, mycoplama, and identity. It is not tested for any specific expression. The depositor listed in Prof H Harris/Dr C Madin, Sir William Dunn School of Pathology, Oxford. The line has not been further modified. Various publications suggest that the E6.1 does express CD28, however we cannot validate these claims. It may be helpful to refer to the literature for further information.
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About This Item
UNSPSC Code:
41106514
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Product Name
Jurkat E6.1 Cell Line human, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88042803, human blood (leukemic T-cell lymphoblast), Not specified
biological source
human blood (leukemic T-cell lymphoblast)
packaging
tube of 5 μg 88042803-DNA-5UG
pkg of vial of cells 88042803-1VL
growth mode
Suspension
karyotype
Pseudodiploid, modal No 46
morphology
Not specified
products
Interleukin 2 (IL-2), human alpha interferon
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
IL-2 assay and cloning studies
Jurkat E6.1 cell line has been used to:
- study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate[1]
- determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components[2]
- study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro[3]
Biochem/physiol Actions
Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.
Human leukaemic T cell lymphoblast
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
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Protocols
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
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Does this particular batch of Jurkat E6.1 express CD28?
1 answer-
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I am interested in Jurkat cells; product number 88042803 appears to be a vial of these cells for culturing. But why is the extension '-DNA-5UG' in the product number? Does this mean that it is not cells but 5 µg DNA isolated from Jurkat cells???
1 answer-
To order the actual cells, order the SKU with the -1VL suffix, 88042803-1VL. This SKU is for the Jurkat E6.1 cells that can be propagated and maintained in culture. The other SKUs listed there are preparations of DNA, RNA and cDNA from this cell line.
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