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H8264

Hanks′ Balanced Salt solution

Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

HBSS

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100 mL

Available to ship TODAYfromMILWAUKEE

$11.80
500 mL

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$33.60
1 L

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$60.40
6 x 500 mL
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$175.00
6 x 1 L

Estimated to ship onNovember 30, 2026fromMILWAUKEE

$317.00

About This Item

NACRES:
NA.75
UNSPSC Code:
12352207

$11.80


Available to ship TODAYDetails


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Quality Segment

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: no
glucose: 1.0 g/L (Dextro)
NaHCO3: 0.35 g/L

shipped in

ambient

storage temp.

room temp

General description

Hanks’ Balanced Salt solution (HBSS) is a buffer that mimics biological medium and stabilizes cells. It is useful in leucocyte labeling studies. HBSS is an effective preservative for periodontal ligament cells. HBSS lacks essential inorganic ions and is useful in starvation studies.

Application

Calcium and magnesium are included in this formulation and support cell adhesion. Used to minimize cell attachment or aggregation.


Hanks’ Balanced Salt solution has been used:

  • as a rinse solution for hepatocytes
  • for hippocampi dissection
  • in corrosion testing of dog-bone specimens
  • for starvation studies in breast cancer cell line
  • as a component of dissociation solution for dorsal root ganglion
  • as a chamber solution for the patch-clamp apparatus

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.

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H6648H9394H4385
description

Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

description

Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

description

Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

description

10 ×, Modified, without calcium, magnesium or sodium bicarbonate

form

liquid

form

liquid

form

liquid

form

-

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

components

phenol red: no
glucose: 1.0 g/L (Dextro)
NaHCO3: 0.35 g/L

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: no

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L

components

-

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

-


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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

  1. Can this be used for testing bioglass in terms of hydroxyapatite growth?

    1 answer
    1. This product has not been tested for the use in bioglass in terms of hydroxyapatite growth. Application specific use of this product needs to be determined by the end user. The formulations of this and other HBSS products are provided below for further evaluation: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/555/001/h8264for.pdf

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