Endotoxin-free products are all typically filtered with a 0.1 micron pore size filter. Sterile filtered products typically have a 0.2 or 0.22 pore size filter.
R8758
RPMI-1640 Medium
With ʟ-glutamine, sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Roswell Park Memorial Institute 1640 medium
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| Size/SKU | Availability | Price |
|---|---|---|
100 mL | Available to ship TODAYfromMILWAUKEE | $17.70 |
500 mL | Available to ship TODAYfromMILWAUKEE | $38.90 |
1 L | Available to ship TODAYfromMILWAUKEE | $62.10 |
6 x 500 mL | Check Cart for Availability | $202.00 |
6 x 1 L | Check Cart for Availability | $335.00 |
24 x 500 mL | Check Cart for Availability | $729.00 |
About This Item
Product Name
RPMI-1640 Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
Application
1 of 1
This Item | |||
|---|---|---|---|
| description With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | description Dutch Modification, with sodium bicarbonate and 20mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture | description Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture | description With L-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture |
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility - |
| form liquid | form liquid | form powder | form powder |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| components NaHCO3: yes | components sodium pyruvate: no | components HEPES: no | components L-glutamine: yes |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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When the medium waas sterile-filtered, which pore size was used?
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Good Morning, does RPMI medium R8758 which contains sodium bicarbonate, affect cell suspension culture of megakaryoblastic cell line DAMI? I mean, sodium bicarbonate can significantly modify cell behavior or his effects can be tolerated?
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This product has not been validated for use with the megakaryoblastic cell line DAMI. However, literature indicates compatibility with this cell line. The following citation may be of interest: PLoS One. 2020 Apr 7;15(4):e0230507.
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I bought this product and realized that it does not contain Hepes. Can I add hepes?
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This product utilizes a bicarbonate buffering system. However, there are no anticipated problems with the addition of HEPES. We do offer RPMI formulations containing both sodium bicarbonate and HEPES, such as Products R5886 & R7638.
Please see the RPMI formulation tables at the link below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/203/777/r8758for.pdf
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Considering that I have to use an RPMI medium containing 4mM of l-Glutamine and 10mM of non-essential amino acids, can I use the product R8748 without adding anything?
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The information you are requesting can be supplied by our Technical Service team who can assist you further. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
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buenos dias, llevo años usando vuestro producto RPMI 1640, y me gustaría saber la composición del medio para ver si hay algun metal/producto que no puede utilizar con un suplemento que quiero introducir. Muchas gracias
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Please see the link below to review the RPMI formulation tables. This product is listed in the second column of page 2:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/203/777/r8758for.pdfHelpful?
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Does Product R8758, RPMI-1640 Medium, contain HEPES?
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There is no HEPES in this formulation of RPMI. We do offer RPMI containing HEPES as Product No's. R5886 and R4130. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.
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Does Product R8758, RPMI-1640 Medium, contain glucose?
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RPMI contains glucose at 2 g/L. The complete formulation of RPMI and other classical media formulations can be found on the Cell Culture Web Page.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Does Product R8758, RPMI-1640 Medium, contain phenol red?
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This media contains phenol red. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.
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Can Product R8758, RPMI-1640 Medium, be frozen?
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We do not recommend freezing cell culture media. A precipitate may form upon thawing. Also, this can cause a loss of amino acids, which may yield low cell growth.
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