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14-383

Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg

Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.

Synonym(s):

Rho Assay Reagent, Rho Detection Reagent, Rhotekin RBD Assay Reagent

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Size/SKUAvailabilityPrice
650 μg
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$662.00

About This Item

UNSPSC Code:
12352200
NACRES:
NA.84
eCl@ss:
32160405

$662.00

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biological source

mouse

Quality Segment

100

manufacturer/tradename

Upstate®

technique(s)

affinity binding assay: suitable

NCBI accession no.

NM_001015055.1, NM_001015056.1, NM_033046.2

UniProt accession no.

Q9BST9

shipped in

dry ice

Gene Information

mouse ... RTKN(20166)

General description

GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E.coli and bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rho, but not GDP-Rho from cell lysates.
Product Source: Expressed in E. coli

Physical form

650 µg of recombinant protein in 1 mL glutathione-agarose slurry containing 45mM TRIS, pH 7.5, 0.45% Triton X-100, 135mM NaCl, 4.5mM MgCl2, 0.9mM dithiothreitol, 0.09mM PMSF, 0.9 µg/mL aprotinin, 0.9 µg/mL leupeptin, and 10% glycerol.

Preparation Note

Stable for 6 months at -70°C from date of receipt.

Analysis Note

Routinely evaluated by precipitating GTP-Rho from 3T3/A31 lysates, subsequent detection by immunoblot analysis using Anti-RhoA, clone 55 (05-778).

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
144551729414415
Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.

Sigma-Aldrich

14383

Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg

Rap1 Assay Reagent (Ral GDS-RBD, agarose), 650 µg Reagent (Ral GDS-RBD, agarose). A GST-tagged fusion protein, corresponding to amino acids 788-884 of human Ral GDS-Rap binding domain (RBD), for use in Affinity Binding Assays.

Sigma-Aldrich

14455

Rap1 Assay Reagent (Ral GDS-RBD, agarose), 650 µg

Rho Activation Assay Kit PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

Sigma-Aldrich

17294

Rho Activation Assay Kit

Ral Assay Reagent (Ral BP1, agarose)

Sigma-Aldrich

14415

Ral Assay Reagent (Ral BP1, agarose)

description

Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.

description

Reagent (Ral GDS-RBD, agarose). A GST-tagged fusion protein, corresponding to amino acids 788-884 of human Ral GDS-Rap binding domain (RBD), for use in Affinity Binding Assays.

description

PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

description

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

biological source

mouse

biological source

human

biological source

-

biological source

-

UniProt accession no.

Q9BST9

UniProt accession no.

Q9NYB0

UniProt accession no.

P08134, P61586, P62745

UniProt accession no.

Q15311

NCBI accession no.

NM_001015055.1, NM_033046.2, NM_001015056.1

NCBI accession no.

NM_018975.2

NCBI accession no.

NM_001664.2

NCBI accession no.

NM_006788.3

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Storage Class

10 - Combustible liquids

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1

wgk

WGK 2

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Certificates of Analysis (COA)

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