14-415

Ral Assay Reagent (Ral BP1, agarose)

• Synonym(s): Agarose Ral Assay, Ral BP1 Assay Reagent, Ral Detection Reagent
• UNSPSC Code: 12352204
• eCl@ss: 32160405
• NACRES: NA.41

Properties

• form: liquid
• Quality Level: 100
• manufacturer/tradename: Upstate^®
• technique(s): affinity binding assay: suitable
• NCBI accession no.: NM_006788.3
• UniProt accession no.: Q15311
• shipped in: dry ice

Description

General description

GST fusion-protein, corresponding to residues 397-519 of rat Ral binding protein (RalBP1) bound to agarose. Also referred to as RLIP and RLP; specifically binds to and precipitates RalA-GTP and RalB-GTP from cell lysates.

Product Source: Expressed in E. coli.

Physical form

300 μg of protein in 600 μL of a 50% agarose slurry in 50 mM Tris, pH 7.5, 200 mM NaCl, 1% Triton X-100, 20 μM PMSF, 1 mM aprotinin prior to the addition of glycerol to 50%. Liquid suspension.

Preparation Note

1 year at -20°C

Analysis Note

Routinely evaluated by Affinity Precipitation/Immunoblot Analysis

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2