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268298

DAPI, Dihydrochloride

Cell-permeable DNA-binding dye.

Synonym(s):

DAPI, Dihydrochloride, 4ʹ,6-Diamidino-2-phenylindole, 2HCl

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Size/SKUAvailabilityPrice
10 mg
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$247.00

About This Item

Empirical Formula (Hill Notation):
C16H15N5 · 2HCl
CAS Number:
28718-90-3
Molecular Weight:
350.25
UNSPSC Code:
12352200
NACRES:
NA.54
MDL number:
MFCD00012681

$247.00

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Quality Segment

100

description

RTECS - NL5995050

assay

≥90% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

color

yellow

solubility

water: 5 mg/mL, ethanol: soluble, methanol: soluble

fluorescence

λex ~359 nm, λem ~461 nm

shipped in

ambient

storage temp.

−20°C

SMILES string

[Cl-].[Cl-].[nH]1c2c(cc1c3ccc(cc3)C(=N)N)ccc(c2)C(=N)N.[H+].[H+]

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

General description

Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H2O (Cat. No. 508741).
Fluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Binds preferentially to DNA rich in adenine and thymine
Product does not compete with ATP.
Reversible: yes

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item
10236276001D9542D8417
DAPI, Dihydrochloride Cell-permeable DNA-binding dye.

Sigma-Aldrich

268298

DAPI, Dihydrochloride

DAPI 4′,6-Diamidine-2′-phenylindole dihydrochloride

Roche

10236276001

DAPI

DAPI for nucleic acid staining

Sigma-Aldrich

D9542

DAPI

4′,6-Diamidino-2-phenylindole dihydrochloride powder, BioReagent, suitable for cell culture, ≥98% (HPLC), suitable for fluorescence

Sigma-Aldrich

D8417

4′,6-Diamidino-2-phenylindole dihydrochloride

description

Cell-permeable DNA-binding dye.

description

4′,6-Diamidine-2′-phenylindole dihydrochloride

description

for nucleic acid staining

description

powder, BioReagent, suitable for cell culture, ≥98% (HPLC), suitable for fluorescence

color

yellow

color

-

color

-

color

-

fluorescence

λem ~461 nm, λex ~359 nm

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 340 nm; λem 488 nm (nur DAPI)

fluorescence

λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 340 nm; λem 488 nm (nur DAPI)

solubility

water: 5 mg/mL, ethanol: soluble, methanol: soluble

solubility

-

solubility

PBS: insoluble, H2O: 20 mg/mL

solubility

PBS: insoluble, H2O: 20 mg/mL

form

solid

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

room temp

storage temp.

2-8°C

storage temp.

2-8°C

assay

≥90% (HPLC)

assay

>90%

assay

≥98% (HPLC)

assay

≥98% (HPLC)

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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