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03708152001

Roche

Streptavidin Mutein Matrix

Streptavidin, suitable for purification of biotinylated proteins and other biomolecules.

Synonym(s):

Biotin-binding Affinity Resin, Reversible Streptavidin Matrix, Streptavidin Beads

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Size/SKUAvailabilityPrice
5 mL

Available to ship TODAYfromMILWAUKEE

$544.00
$478.72

About This Item

NACRES:
NA.21
UNSPSC Code:
12352200
Form:
suspension
Mol wt:
60 kDa (approx.)

$478.72

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form

suspension

mol wt

60 kDa (approx.)

packaging

pkg of 5 mL

manufacturer/tradename

Roche

parameter

4-25 °C optimum reaction temp.

matrix

(immobilised onto crosslinked agarose beads)

shipped in

wet ice

storage temp.

2-8°C

General description

Contents
Settled resin of 50% suspension Streptavidin Mutein Matrix.
Streptavidin mutein, immobilized.
The binding of biotin to avidin/streptavidin is the strongest known noncovalent interaction of biological molecules [(Kd 0.6 x 10-15 M (avidin), 4 x 10-14 M (streptavidin)]. Strong binding provides a great advantage for certain applications of the derived biotin-avidin/streptavidin technology, such as surface immobilization, blotting technologies, immunoassays, cross-linking studies, and histochemistry. However, the use of immobilized avidin/streptavidin for the purification of biotinylated proteins is limited. In this instance, the extreme affinity of the binding partners creates a drawback of enabling elution only under very harsh conditions that usually destroy the functionality of the protein of interest.
Recombinant streptavidin technology was used to screen for a mutant streptavidin with reduced binding affinity toward biotin. By substituting three amino acids, a mutein was obtained with a biotin dissociation constant of 1.3 x 10-7 M.
The Streptavidin Mutein Matrix provides this mutein immobilized onto cross-linked agarose beads in a highly stable and regenerable form, which allows purification of biotinylated proteins that results in excellent purity and recovery.

Application

  • Streptavidin Mutein Matrix: Is highly suitable for purification of biotinylated proteins
  • Has been tested for use with in vitro expressed biotinylated proteins, using the RTS AviTag Biotinylation Kits
  • Is convenient for purification of chemically biotinylated proteins
  • It was also used in single-step affinity chromatography and affinity chromatography.

Features and Benefits

  • Mild purification conditions: Low biotin dissociation constant allows elution of biotinylated proteins under gentle conditions.
  • Stable: No significant loss of binding capacity upon storage. The low dissociation constant of the streptavidin mutein is obtained by genetic engineering and not by dissociation of avidin- or streptavidin tetramers.
  • Economic: High binding capacity and multiple reuse allows economic handling of the matrix.

Preparation Note

Store at 2 to 8 °C. (Do not freeze!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Streptavidin Mutein Matrix Streptavidin, suitable for purification of biotinylated proteins and other biomolecules.

Roche

03708152001

Streptavidin Mutein Matrix

HiTrap® Streptavidin High Performance Cytiva 17-5112-01, pack of 5 × 1 mL

GE17511201

HiTrap® Streptavidin High Performance

Streptavidin Agarose Column or batch isolation of biotinylated molecules

Millipore

69203

Streptavidin Agarose

Streptavidin Sepharose™ High Performance Cytiva 17-5113-01, pack of 5 mL

GE17511301

Streptavidin Sepharose™ High Performance

description

Streptavidin, suitable for purification of biotinylated proteins and other biomolecules.

description

Cytiva 17-5112-01, pack of 5 × 1 mL

description

Column or batch isolation of biotinylated molecules

description

Cytiva 17-5113-01, pack of 5 mL

form

suspension

form

-

form

liquid

form

resin

mol wt

60 kDa (approx.)

mol wt

-

mol wt

-

mol wt

-

shipped in

wet ice

shipped in

-

shipped in

ambient

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

packaging

pkg of 5 mL

packaging

pack of 5 × 1 mL

packaging

-

packaging

pack of 5 mL

matrix

(immobilised onto crosslinked agarose beads)

matrix

highly cross-linked 6% agarose

matrix

-

matrix

highly cross-linked 6% agarose

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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