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Merck

COLLDISP-RO

Roche

Collagenase/Dispase®

lyophilized, non-sterile, optimum pH 7.0-8.0

Synonim(y):

collagenase

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100 MG

815,00 zł

500 MG

2350,00 zł

815,00 zł

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Informacje o tej pozycji

UNSPSC Code:
12352204
NACRES:
NA.54
Specific activity:
>0.8 U/mg lyophilizate, pH 7.5, 37 °C (casein as substrate, Dispase)
Biological source:
enzyme from Bacillus polymyxa ( Dispase ), enzyme from bacterial ( Collagenase from Vibrio alginolyticus)
Concentration:
1 mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)

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biological source

enzyme from Bacillus polymyxa ( Dispase ), enzyme from bacterial ( Collagenase from Vibrio alginolyticus)

sterility

non-sterile

form

lyophilized

collagenase activity

>0.1 U/mg lyophilizate, 25 °C (4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as substrate)

specific activity

>0.8 U/mg lyophilizate, pH 7.5, 37 °C (casein as substrate, Dispase)

packaging

pkg of 100 mg (10269638001), pkg of 500 mg (11097113001)

manufacturer/tradename

Roche

concentration

1 mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)

technique(s)

tissue processing: suitable

color

light yellow to brown

optimum pH

7.0-8.0

solubility

water: soluble

UniProt accession no.

P43154

application(s)

life science and biopharma
sample preparation

foreign activity

proteolytic activities <0.1 U/mg (hemoglobin as the substrate)

storage temp.

2-8°C

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Ta pozycja
04942078001COLLA-ROCOLLD-RO
Collagenase/Dispase&#174; lyophilized, non-sterile, optimum pH 7.0-8.0

Roche

COLLDISP-RO

Collagenase/Dispase®

Dispase&#174; II (neutral protease, grade II) lyophilized, from bacterial, Roche, pkg of 5 × 1&#160;g

Roche

04942078001

Dispase® II (neutral protease, grade II)

Collagenase A from Clostridium histolyticum

Roche

COLLA-RO

Collagenase A

Collagenase D from Clostridium histolyticum

Roche

COLLD-RO

Collagenase D

biological source

enzyme from Bacillus polymyxa ( Dispase ), enzyme from bacterial ( Collagenase from Vibrio alginolyticus)

biological source

bacterial

biological source

Clostridium histolyticum

biological source

Clostridium histolyticum

technique(s)

tissue processing: suitable

technique(s)

-

technique(s)

-

technique(s)

tissue processing: suitable

specific activity

>0.8 U/mg lyophilizate, pH 7.5, 37 °C (casein as substrate, Dispase)

specific activity

≥0.8 units/mg protein (37 °C, casein as substrate, pH 7.5)

specific activity

>0.15 U/mg lyophilizate (Collagenase activity)

specific activity

-

concentration

1 mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)

concentration

-

concentration

1 mg/mL (0.1%, w/v)

concentration

(working concentration: 0.5 - 2.5 mg/ml)

application(s)

life science and biopharma
sample preparation

application(s)

sample preparation

application(s)

cell analysis
life science and biopharma
sample preparation

application(s)

cell analysis

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

General description

Dispase is a neutral, rapid-acting, gentle protease that separates intact epidermis from dermis. It also separates cultured intact epithelial cells from the substratum. It cleaves the basement membrane zone and leaves the epithelial cells intact.[1] Collagenase is a protease which cleaves the triple-helical protein called collagen. There are three types of tissue collagenases, and these belong to the matrix metalloproteinases (MMP) family.[2] Collagenase obtained from Clostridium histolyticum has a very strong activity, as it digests collagen from both ends, at temperatures as low as 4-10 °C.[3]

Application

Collagenase/Dispase® is used for the preparation of cells from a wide variety of different tissues and organs.[4][5][6]

Biochem/physiol Actions

Collagenase degrades native collagen. Dispase® is a nonspecific protease.

Preparation Note

Activator: Ca2+

Working concentration: Approximately 1mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)

Enzyme activity:
Collagenase: 0.1 Wünsch U/mg lyophilizate (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Dispase®: 0.8 Wünsch U/mg lyophilizate (+37°C, casein as substrate, pH 7.5)
Contaminating proteolytic activities: <0.1U/mg lyophilizate (hemoglobin as the substrate)

Working solution: Preparation of working solution Dissolve enzyme in water to a final concentration of 100mg/mL. Dilute with PBS (Ca2+/Mg2+-free) to a final concentration of 1mg/mL and filter through a sterile 0.2μm membrane filter.
Enzyme concentration of the sterile solution: Collagenase, 0.1U/mL PBS; Dispase®, 0.8U/mL PBS.

Inhibitors:
Collagenase inhibitors: EDTA, EGTA
Collagenase/Dispase is not inhibited by serum.

Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable at -15 to -25°C.
Note: Store in appropriate aliquots and avoid repeated freezing and thawing!
Reconstitution in water (100mg/mL). Further dilution with PBS (phosphate buffered saline) (Ca2+/Mg2+-free).
Store dry

Other Notes

Collagenase from Vibrio alginolyticus, Dispase® from Bacillus polymyxa
EC 3.4.24.4 & 3.4.24.3
For life science research only. Not for use in diagnostic procedures.
One unit is the enzyme activity, which liberates under assay conditions within 1 minute Folin-positive amino acids and peptides corresponding to 1 μmol tyrosine.

Legal Information

Dispase is a registered trademark of Godo Shusei Co., Ltd.
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pictograms

Exclamation markHealth hazard
GHS07,GHS08

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Klasa składowania

11 - Combustible Solids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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Certyfikaty analizy (CoA)

Lot/Batch Number
73680321
68654621
63837023
59985623
57028122

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