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ENDOLYSS-RO

Roche

Endoproteinase Lys-C Sequencing Grade

from Lysobacter enzymogenes

Synonym(s):

lys-c, lysyl endopeptidase, protease

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Pack SizeSKUAvailabilityPrice
5 μg
Please contact Customer Service for Availability
$273.00
3 x 5 μg
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$748.00

About This Item

UNSPSC Code:
12352204
EC Number:
3.4.21.50 (BRENDA, IUBMB)
NACRES:
NA.54
Specific activity:
≥200 units/mg protein (at 25 °C, with Chromozym PL)
Biological source:
bacterial (Lysobacter enzymogenes)

$273.00

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biological source

bacterial (Lysobacter enzymogenes)

form

lyophilized

specific activity

≥200 units/mg protein (at 25 °C, with Chromozym PL)

mol wt

33 kDa by SDS-PAGE (reducing)

purified by

electrophoresis

packaging

pkg of 3 × 5 μg (11047825001), pkg of 5 μg (11420429001)

manufacturer/tradename

Roche

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

technique(s)

activity assay: suitable

color

white

optimum pH

8.5-8.8

pH range

5-12

solubility

water: soluble

suitability

suitable for enzyme test

UniProt accession no.

Q7M135

storage temp.

2-8°C

General description

Endoproteinase Lys-C Sequencing Grade is isolated from Lysobacter enzymogenes as a highly purified and specific protease. Endoproteinase Lys-C from Lysobacter enzymogenes is a member of the peptidase S1 family. It is mostly used for the initial fragmentation of polypeptide chains in protein sequence analysis.

Application

Use Endoproteinase Lys-C Sequencing Grade  for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.[1][2][3][4]

Biochem/physiol Actions

This enzyme is a serine protease that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of Lys (in Tris-HCl buffer, pH 7.0 to 9.0).
Heat inactivation: Enzyme can be denatured using heat (5 min at 100 °C) or by TCA.

Preparation Note

Storage conditions (working solution): +2 to +8 °C
Lyophilized Endoproteinase Lys-C sequencing grade is reconstituted in 50 μl double-dist. water. This results in a buffer concentration of 50 mM Hepes, pH 8.0, 10 mM EDTA and 5 mg/ml raffinose. To avoid autolysis, the incubation temperature should not exceed 37 °C.
Stable in 4 M urea.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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ENDOGLUS-ROLYSC9000LYSC9001
Endoproteinase Lys-C Sequencing Grade from Lysobacter enzymogenes

Roche

ENDOLYSS-RO

Endoproteinase Lys-C Sequencing Grade

Endoproteinase Glu-C Sequencing Grade from Staphylococcus aureus V8

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ENDOGLUS-RO

Endoproteinase Glu-C Sequencing Grade

Lys-c (lysyl-endopeptidase), Active from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), His Tag, solution

Sigma-Aldrich

LYSC9000

Lys-c (lysyl-endopeptidase), Active

Lys-c (lysyl-endopeptidase), Active from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), His-tag, powder

Sigma-Aldrich

LYSC9001

Lys-c (lysyl-endopeptidase), Active

biological source

bacterial (Lysobacter enzymogenes)

biological source

Staphylococcus aureus (V8)

biological source

Achromobacter lyticus

biological source

Achromobacter lyticus

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

specific activity

≥200 units/mg protein (at 25 °C, with Chromozym PL)

specific activity

≥15 units/mg protein

specific activity

-

specific activity

-

suitability

suitable for enzyme test

suitability

-

suitability

-

suitability

-

form

lyophilized

form

lyophilized (salt-free)

form

ready-to-use solution

form

lyophilized powder

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

storage condition

-

storage condition

-

storage condition

-

Storage Class

11 - Combustible Solids

flash_point_f

does not flash

flash_point_c

does not flash

pictograms

Health hazard
GHS08

signalword

Warning

hcodes

H373

Hazard Classifications

STOT RE 2

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
90827421
82646622
73164922
71452526
71059121

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H Alexander Ebhardt et al.
Rapid communications in mass spectrometry : RCM, 28(24), 2735-2743 (2014-11-08)
Tandem mass (MS/MS) spectra generated by collision-induced dissociation (CID) typically lack redundant peptide sequence information in the form of e.g. b- and y-ion series due to frequent use of sequence-specific endopeptidases cleaving C- or N-terminal to Arg or Lys residues.
Heterologous expression and pro-peptide supported refolding of the high specific endopeptidase Lys-C
Stressler T, et al.
Protein Expression and Purification, 118, 31-38 (2016)
Jung Hun Oh et al.
Journal of proteome research, 10(3), 1406-1415 (2011-01-14)
Many efforts have been made to discover novel bio-markers for early disease detection in oncology. However, the lack of efficient computational strategies impedes the discovery of disease-specific biomarkers for better understanding and management of treatment outcomes. In this study, we
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Global Trade Item Number

SKUGTIN
1142042900104061838701831
1104782500104061838250766