This is a solution of 10% safranin in ethanol.
94635
Gram′s safranin solution
suitable for microscopy
Synonym(s):
Safranin T solution
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 250 mL | Check Cart for Availability | $58.70 | |
| 2.5 L | Please contact Customer Service for Availability | $284.00 |
About This Item
Empirical Formula (Hill Notation):
C20H19ClN4
CAS Number:
Molecular Weight:
350.84
UNSPSC Code:
12171500
NACRES:
MA.02
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3924099
form
liquid
Quality Level
shelf life
limited shelf life, expiry date on the label
composition
ethanol, 10%
technique(s)
microbe id | staining: suitable
color
red to very dark red
refractive index
n20/D 1.339
solubility
water: soluble at 20 °C
density
0.986 g/mL at 20 °C
εmax
4.0 at 532 nm in 50% ethanol
suitability
suitable for microscopy
antibiotic activity spectrum
Gram-negative bacteria, Gram-positive bacteria
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
InChI
1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
InChI key
OARRHUQTFTUEOS-UHFFFAOYSA-N
General description
Gram’s safranin solution, also known as Safranin T or Basic Red 2, is a ready-to-use staining solution that comprises 10% safranin in ethanol. Safranin is a basic, cationic, azine dye containing a planar, diaminophenazine chromophore, with a phenyl substituent attached to a ring nitrogen. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. It is most notably used in bacteriology as a counterstain in Gram staining to differentiate between Gram-positive and Gram-negative bacteria.
Application
Gram’s safranin solution has been used in the following applications:
- as a non-toxic replacement for crystal violet for the quantification of biofilm formation
- to develop a hyaluronic acid visualization method
- to study improved β-lactam susceptibility against biofilm-embedded staphylococcus aureus by 2-aminothiazole
Biochem/physiol Actions
Gram staining enables the differentiation of bacteria into Gram-positive and Gram-negative. The mureine structure of the bacterial cell wall is the basis of the color affinity. Bacteria are first stained with crystal violet, and then with iodine solution, which forms a dye-iodine complex. This complex is retained in the multi-layer mureine structure of the cell wall of Gram-positive bacteria after decolorization, imparting a blue-violet color. The cell wall of Gram-negative bacteria comprises a single-layered murein structure that does not retain the dye-iodine complex, allowing the uptake of safranine counterstain, and imparting a pink-red color.
Features and Benefits
- Ready-to-use solution.
- Modified and designed in such a way that staining can be carried out in staining cells, on the staining rack, and in automated staining systems.
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This Item | |||
|---|---|---|---|
| suitability suitable for microscopy | suitability suitable for microscopy | suitability - | suitability suitable for microscopy |
| technique(s) microbe id | staining: suitable | technique(s) titration: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable |
| color red to very dark red | color - | color green to very dark green | color - |
| solubility water: soluble at 20 °C | solubility methanol: 0.01 g/10 mL, red | solubility H2O: 1 mg/mL | solubility - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form liquid | form powder or crystals | form powder | form liquid |
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
flash_point_f
114.8 °F
flash_point_c
46 °C
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 94635-2.5L-F | 04061833295991 |
| 94635-250ML-F | 04061833296042 |