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30674

Atto 700

BioReagent, suitable for fluorescence, ≥90.0% (HPCE)

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1 mg

Available to ship TODAYfromMILWAUKEE

$319.00
$239.25

About This Item

UNSPSC Code:
41116100
NACRES:
NA.32
MDL number:
MFCD06798563

$239.25

List Price$319.00Save 25%
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product line

BioReagent

Quality Segment

100

assay

≥90.0% (HPCE)

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm, 700 nm

fluorescence

λex 681 nm; λem 714 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid: 692 nm ± 3 nm)

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 700 is a new label with high molecular absorption (1.2 x 105) and quantum yield (0.25) as well as sufficient Stoke′s shift between excitation and emission maximum. Atto 700 is characterized by high photostability and thermal stability. It is also excellently soluble in water. Due to an insignificant triplet formation rate, it is well suited for single molecule detection applications.

Application

Atto 700 belongs to a new generation of fluorescent labels. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, excellent thermal and photo-stability, very good water solubility and very little triplet formation. Atto 700 is a zwitterionic dye with a net electrical charge of zero. The fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc.
Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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This Item
744176745552595
Atto 700 BioReagent, suitable for fluorescence, ≥90.0% (HPCE)

Sigma-Aldrich

30674

Atto 700

Sigma-Aldrich

Sigma-Aldrich

74417

Atto 488 amine

Sigma-Aldrich

Sigma-Aldrich

67455

Atto 514 NHS ester

Sigma-Aldrich

Sigma-Aldrich

52595

Atto 514

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

fluorescence

λex 681 nm; λem 714 nm in 0.1 M phosphate pH 7.0

fluorescence

-

fluorescence

λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4

fluorescence

λex 511 nm; λem 533 nm±10 nm in PBS, pH 7.4

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

-

assay

≥90.0% (HPCE)

assay

≥90% (HPLC)

assay

90% (Coupling efficiency with benzylamine, HPLC)

assay

≥90.0% (HPLC)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

product line

BioReagent

product line

BioReagent

product line

-

product line

-

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP0742
BCCN2272
BCCM9223
BCCM4965
BCCL4374

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Articles

Fluorescence Lifetime Measurement

Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

Multicolour single molecule imaging in cells with near infra-red dyes.
Tynan, C.J., et al.
PLoS ONE, 4, e36265-e36265 (2012)
Daniel Riester et al.
Bioorganic & medicinal chemistry letters, 19(13), 3651-3656 (2009-05-22)
Histone deacetylases reside among the most important and novel target classes in oncology. Selective lead structures are intensively developed to improve efficacy and reduce adverse effects. The common assays used so far to identify new lead structures suffer from many
Judith E Berlier et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(12), 1699-1712 (2003-11-19)
Amine-reactive N-hydroxysuccinimidyl esters of Alexa Fluor fluorescent dyes with principal absorption maxima at about 555 nm, 633 nm, 647 nm, 660 nm, 680 nm, 700 nm, and 750 nm were conjugated to antibodies and other selected proteins. These conjugates were
View All Related Papers

Global Trade Item Number

SKUGTIN
30674-1MG-F04061826750087

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