If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
43815
DL -Dithiothréitol
BioUltra, Molecular Biology, ≥99.5% (RT)
Synonyme(s) :
(±)-Dithiothréitol, rac-Dithiothréitol, Dithiothréitol, thréo-1,4-Dimercapto-2,3-butanediol, DTT, Réactif de Cleland
Sélectionner une taille de conditionnement
| À vous/Référence | Disponibilité | Prix |
|---|---|---|
1 g | Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit. | 142,00 € |
5 g | Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit. | 491,00 € |
25 g | Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit. | 1 920,00 € |
A propos de cet article
142,00 €
grade
Molecular Biology
Quality Segment
product line
BioUltra
assay
≥99.5% (RT)
form
powder
reaction suitability
reagent type: reductant
impurities
DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.3% 4,5-dihydroxy-1,2-dithiane
pH
4.0-6.5 (25 °C, 0.1 M in H2O)
mp
41-44 °C (lit.)
solubility
H2O: 0.1 M at 20 °C, clear, colorless
anion traces
sulfate (SO42-): ≤50 mg/kg
cation traces
Al: ≤5 mg/kg, As: ≤0.5 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤500 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
λ
0.1 M in H2O
UV absorption
λ: 260 nm Amax: 0.400, λ: 280 nm Amax: 0.100
SMILES string
O[C@H](CS)[C@H](O)CS
storage temp.
2-8°C
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key
VHJLVAABSRFDPM-QWWZWVQMSA-N
General description
Application
- comme stabilisant d'enzyme pour maintenir la stabilité de l'exebacase[1].
- dans l'extraction de peptides et la dénaturation de protéines.
- dans la préparation d'échantillons et la rupture des liaisons de réticulation par le formaldéhyde avant spectrométrie de masse[2].
- comme constituant d'un tampon pour l'extraction de synaptogliosome.
Biochem/physiol Actions
Features and Benefits
- Poudre de qualité BioUltra convenant à la biologie moléculaire.
- Produit exempt de DNases, RNases, phosphatases et protéases. Fraction insoluble : satisfait au test de filtration.
- Produit testé de manière rigoureuse et dépourvu de métaux à l'état de traces.
Other Notes
1 of 1
Cet article | |||
|---|---|---|---|
| grade Molecular Biology | grade Molecular Biology | grade - | grade - |
| assay ≥99.5% (RT) | assay ≥98% (HPLC), ≥99% (titration) | assay ≥99.0% (titration) | assay ≥99.0% (RT) |
| product line BioUltra | product line - | product line BioXtra | product line - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility H2O: 0.1 M at 20 °C, clear, colorless | solubility H2O: 50 mg/mL | solubility H2O: 0.1 M, clear, colorless | solubility H2O: 50 mg/mL, clear, colorless, H2O: freely soluble, acetone: freely soluble, chloroform: freely soluble, diethyl ether: freely soluble, ethanol: freely soluble, ethyl acetate: freely soluble |
| impurities DNases, none detected, insoluble matter, passes filter test, proteases, none detected, RNases, none detected, ≤0.3% 4,5-dihydroxy-1,2-dithiane, phosphatases, none detected | impurities - | impurities Insoluble matter, passes filter test, ≤0.3% 4,5-dihydroxy-1,2-dithiane | impurities ≤0.3% 4,5-dihydroxy-1,2-dithiane |
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Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
Classe de stockage
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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Is it normal for an IgG1 solution to become milky after DTT reduction?
1 answer-
It is uncommon for a reducing agent to cause protein precipitation. However, in some cases, the reduction of a protein with a reducing agent like DTT can result in a change in the isoelectric point of the protein or protein fragments. This change in pH may create conditions where precipitation of the protein is more likely to occur.
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