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Atto 590 NHS ester

Name: Atto 590 NHS ester
Brand: SIGMA
Price: 159 USD
Availability: InStock

BioReagent, suitable for fluorescence, ≥90.0% (degree of coupling)

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Pack Size	SKU	Availability	Price
1 mg		Check Cart for Availability	$159.00
5 mg		Check Cart for Availability	$897.00

About This Item

Empirical Formula (Hill Notation):

C₄₁H₄₂ClN₃O₁₁

CAS Number:

670269-33-7

Molecular Weight:

788.24

UNSPSC Code:

12352108

NACRES:

NA.32

MDL number:

MFCD03456133

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Properties

product line

BioReagent

Quality Segment

100

assay

≥90.0% (HPLC), ≥90.0% (degree of coupling)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0,1% trifluoroacetic acid)

UV absorption

λ: 597-663 nm A_max

suitability

suitable for fluorescence

detection method

fluorometric

storage temp.

−20°C

SMILES string

[Cl](=O)(=O)(=O)[O-].[N+]1(=C2C(=CC3=C(c6c(cc7c(c6)C(=CC(N7CC)(C)C)C)OC3=C2)c4c(ccc(c4)C(=O)ON5C(=O)CCC5=O)C(=O)O)C(=CC1(C)C)C)CC

InChI

1S/C41H41N3O7.ClHO4/c1-9-42-31-18-33-29(16-26(31)22(3)20-40(42,5)6)37(30-17-27-23(4)21-41(7,8)43(10-2)32(27)19-34(30)50-33)28-15-24(11-12-25(28)38(47)48)39(49)51-44-35(45)13-14-36(44)46;2-1(3,4)5/h11-12,15-21H,9-10,13-14H2,1-8H3;(H,2,3,4,5)

InChI key

SHJDVERDESTYRQ-UHFFFAOYSA-N

Description

General description

Atto 590 is a novel fluorescent label belonging to the class of Rhodamine dyes. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. The Atto dyes are a series of fluorescent dyes that provide all the crucial properties required for modern fluorescent technologies, such as fluorescence microscopy, flow-cytometry, fluorescence in situ hybridization (FISH), receptor binding assays or enzyme assays. The dye is highly suitable for single-molecule detection applications and high-resolution microscopy. The NHS-esters are used in common conjugation protocols.

Application

Atto 590 NHS ester may be suitable for use in site-specific labeling of human embryonic kidney (HEK293T) cell lysates for western blotting, fluorescence, and widefield microscopy studies.[1]

Molar absorption 120,000 1/M cm, abs: 593 nm, em: 620 nm, QY=0.93, T_fl 4.0 ns (unpublished data)

Features and Benefits

Absorption maxima cover a range from 590 to 620 nm.
Structural properties support extraordinary photostability.
High fluorescence quantum yield and high thermal stability.
The fluorescence characteristics of the dye are quite insensitive to environmental changes (e.g., pH changes). This holds for both excitation and emission wavelengths and for the decay time of their fluorescence emission.
Compatible with common labeling procedures for proteins and amino-functionalized oligonucleotides.

Other Notes

Study of multistep energy transfer in a single photonic wire by FRET[2]; employed in fluorescence energy transfer, FRET, scanning microscopy.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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1 of 1

This Item	67455	78362	68606
Supelco 79636 Atto 590 NHS ester	Sigma-Aldrich 67455 Atto 514 NHS ester	Sigma-Aldrich 78362 Atto 490LS NHS ester	Sigma-Aldrich 68606 Atto 594-Streptavidin
suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence	suitability -
form powder	form solid	form solid	form solid
assay ≥90.0% (HPLC), ≥90.0% (degree of coupling)	assay 90% (Coupling efficiency with benzylamine, HPLC)	assay ≥90% (HPLC), ≥90% (degree of coupling)	assay -
Quality Level 100	Quality Level -	Quality Level 100	Quality Level 100
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
product line BioReagent	product line -	product line BioReagent	product line BioReagent

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Global Trade Item Number

SKU	GTIN
79636-1MG-F	04061826270882
79636-5MG-F	04061832533193

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