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87444

5-Carboxy-fluorescein diacetate N-succinimidyl ester

suitable for fluorescence, ≥95.0% (HPLC)

Synonym(s):

5-Carboxy-di-O-acetylfluorescein N-succinimidyl ester

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Pack SizeSKUAvailabilityPrice
5 mg
Please contact Customer Service for Availability
$262.00
$183.40

About This Item

Empirical Formula (Hill Notation):
C29H19NO11
CAS Number:
150206-05-6
Molecular Weight:
557.46
NACRES:
NA.32
PubChem Substance ID:
57653248
UNSPSC Code:
12352108
MDL number:
MFCD00037466

$183.40

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Quality Level

100

assay

≥95.0% (HPLC)

form

powder

solubility

DMF: soluble, DMSO: soluble

fluorescence

λex 492 nm; λem 517 nm

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7

InChI

1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3

InChI key

LZFDYQHOQDFMOA-UHFFFAOYSA-N

Other Notes

Continuous determination of the intracellular pH in bacteria by fluorometry[1]; labeling human intervertebral disk cells in vitro for fluorescence microscopy[2]

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This Item
C50412187946940
5-Carboxy-fluorescein diacetate N-succinimidyl ester suitable for fluorescence, ≥95.0% (HPLC)

Sigma-Aldrich

87444

5-Carboxy-fluorescein diacetate N-succinimidyl ester

6-Carboxyfluorescein diacetate ≥95% (HPLC)

Sigma-Aldrich

C5041

6-Carboxyfluorescein diacetate

5(6)-Carboxyfluorescein diacetate BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

Sigma-Aldrich

21879

5(6)-Carboxyfluorescein diacetate

6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester suitable for fluorescence, ≥75% (HPLC)

Sigma-Aldrich

46940

6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester

suitability

suitable for fluorescence

suitability

-

suitability

suitable for fluorescence

suitability

suitable for fluorescence

fluorescence

λex 492 nm; λem 517 nm

fluorescence

-

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

fluorescence

λex 490 nm; λem 514 nm (pH 8.2), λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0

solubility

DMF: soluble, DMSO: soluble

solubility

chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow

solubility

DMSO: soluble

solubility

DMF: soluble, methanol: soluble

form

powder

form

powder

form

powder

form

solid

assay

≥95.0% (HPLC)

assay

≥95% (HPLC)

assay

≥90.0% (HPLC)

assay

≥75% (HPLC)

Quality Level

100

Quality Level

100, 200

Quality Level

100

Quality Level

100

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN5406
BCCL9002
BCCH7934
BCCJ7672
BCCD0002

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Juan Dubrot et al.
The Journal of experimental medicine, 211(6), 1153-1166 (2014-05-21)
Dendritic cells (DCs), and more recently lymph node stromal cells (LNSCs), have been described to tolerize self-reactive CD8(+) T cells in LNs. Although LNSCs express MHCII, it is unknown whether they can also impact CD4(+) T cell functions. We show
H E Gruber et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 75(3), 118-123 (2000-08-19)
We have assessed the utility of an intracellular fluorochrome, 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE), as a tracking label for human intervertebral disc cells in vitro. Although 5 microM provides adequate intracellular labeling for whole cell fluorescent microscopic identification of labeled
P Breeuwer et al.
Applied and environmental microbiology, 62(1), 178-183 (1996-01-01)
A novel method based on the intracellular conjugation of the fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester (cFSE) was developed to determine the intracellular pH of bacteria. cFSE can be taken up by bacteria in the form of its diacetate
View All Related Papers

Global Trade Item Number

SKUGTIN
87444-5MG-F04061832533421

Questions

1–3 of 3 Questions  

  1. Can user the product 5-Carboxy-fluorescein diacetate N-succinimidyl ester 87444 in cells living infected with parasite?

    1 answer

    1. This application will need to be validated by the end user. This product is passively taken up by cells. Fluorescence occurs when the dye is hydrolyzed by esterases within the cell. The dye reacts covalently with amine groups present on intercellular proteins, which allows for long-term staining effects. This dye may be taken up by the parasite, as well.

      The link below may offer additional helpful information:
      https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/carboxyfluorescein-diacetate-succinimidyl-ester

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. Can product 87444, 5-Carboxy-fluorescein diacetate N-succinimidyl ester, be used in flow cytometry?

    1 answer

    1. Yes, product 87444, 5-Carboxy-fluorescein diacetate N-succinimidyl ester, can be used in flow cytometry. The optimal concentration range for cell labeling is 2.5 uM to10 M. The concentration of the cell line will have to be optimized.

      Helpful?

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