This product can be used at room temperature.
R4533
RNAzol® RT
For processing total and small RNA from human, animal, plant, bacterial,and viral samples
Synonym(s):
single step RNA isolation, single step RNA purification, total RNA isolation, total RNA purification
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| Size/SKU | Availability | Price |
|---|---|---|
50 mL | Available to ship TODAYfromMILWAUKEE | $147.00 |
100 mL | Available to ship TODAYfromMILWAUKEE | $257.00 |
200 mL | Available to ship TODAYfromMILWAUKEE | $436.00 |
About This Item
NACRES:
NA.52
UNSPSC Code:
12352200
$147.00
Available to ship TODAYDetails
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Quality Segment
usage
mL sufficient for 107 cells, mL sufficient for 100 mg tissue (or)
technique(s)
whole genome amplification: suitable
General description
RNAzol RT is a quick and convenient reagent for use in the single-step isolation of total and small RNA from biological samples of human, animal, plant, yeast, bacterial, and viral origin. A convenient single-step liquid phase separation results in the isolation of RNA from DNA, protein, polysaccharides, and other molecules. RNAzol® RT can be used to isolate separate fractions of mRNA and micro RNA or to isolate total RNA, containing all classes of RNA in a single fraction.
This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. Addition of water to the mixture allows for the precipitation of DNA, proteins, polysaccharides and other molecules, which can then be removed by centrifugation. RNA can then be isolated by alcohol precipitation, washing and solubilization. Chloroform-induced phase separation is not necessary. One mL of RNAzol® RT is sufficient to isolate RNA from up to 100 mg of tissue or 10e7 cells.
This is one of the most effective methods for isolating total and small RNA and can be completed at room temperature in less than 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types: large nuclear RNA, rRNA, mRNA, small RNA and micro RNA. The resulting RNA is intact with little or no contaminating DNA and protein.
This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. Addition of water to the mixture allows for the precipitation of DNA, proteins, polysaccharides and other molecules, which can then be removed by centrifugation. RNA can then be isolated by alcohol precipitation, washing and solubilization. Chloroform-induced phase separation is not necessary. One mL of RNAzol® RT is sufficient to isolate RNA from up to 100 mg of tissue or 10e7 cells.
This is one of the most effective methods for isolating total and small RNA and can be completed at room temperature in less than 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types: large nuclear RNA, rRNA, mRNA, small RNA and micro RNA. The resulting RNA is intact with little or no contaminating DNA and protein.
Application
Isolated RNA can be used for Northern blots, RNase protection assay, microarrays, PCR, and other molecular biology applications.
RNAzol RT is a highly effective reagent for the single-step isolation of total and small RNA from human, animal, plant, bacterial and viral origin samples. The RNA isolation method based on this reagent is performed at room temperature, without the use of chloroform for phase separation, and allows for use in RT-PCR without requiring DNase treatment.
Features and Benefits
- Isolates micro RNA and total RNA isolation of pure and undegraded RNA from biological samples
- High yields and increased quality of isolated RNA
- Completed at room temperature in less than an hour starting with fresh tissue or cells
Legal Information
RNAZol is a registered trademark of Molecular Research Center, Inc.
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This Item | |||
|---|---|---|---|
| description For processing total and small RNA from human, animal, plant, bacterial,and viral samples | description - | description LS, For processing fluid samples such as cell suspensions, CSF, and amniotic fluid. | description solution (ready-to-use), pkg of 50 mL (11667157001), pkg of 200 mL (11667165001) |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level - |
| usage mL sufficient for 107 cells, mL sufficient for 100 mg tissue (or) | usage mL sufficient for 107 cells, mL sufficient for 100 mg tissue | usage 0.75 mL sufficient for 0.25 mL fluid samples | usage - |
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
target_organs
Nervous system,Kidney,Liver,Skin
Storage Class
8A - Combustible corrosive hazardous materials
wgk
WGK 3
flash_point_f
230.0 °F
flash_point_c
110 °C
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Can RNAzol RT be used at room temperature or must it be cooled to 4°C before lysing cell pellets?
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What color is Product R4533, RNAzol® RT?
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Product R4533, RNAzol® RT is a blue solution at both 4°C and room temperature.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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