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A0705

Anti-Bovine IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody

Synonym(s):

Rabbit Anti-Bovine IgG (whole molecule)−AP

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0.25 mL
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$315.00
1 mL
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$980.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162168
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d)
Citations:
17

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biological source

rabbit

Quality Segment

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

bovine

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Immunoglobulins (Igs) belongs to immunoglobulin super-family (IgSF). Igs in general, consist of two heavy (H) chains and two light (L) chains. The L chain can be either κ or a λ chain. Each chain contains one NH2-terminal “variable" (V) region and one or more COOH-terminal “constant” (C) regions. Each of which consists of two sandwiched β pleated sheets ′pinned′ together by a disulfide bridge. There are five main classes of Igs based on heavy chain C domains, they are, IgM, IgG, IgA, IgD, and IgE isotypes. IgG can be divided into four subclasses, IgG1, IgG2, IgG3, and IgG4. Each subclass possesses distinct biologic properties.

Immunogen

Purified bovine IgG

Application

Anti-Bovine IgG (whole molecule)-Alkaline Phosphatase antibody produced in rabbit has been used in
  • enzyme linked immunosorbent assay (ELISA)
  • development of immunosensors
  • western blot analysis

Biochem/physiol Actions

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin [1]. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration [2].

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Bovine IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit affinity isolated antibody

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Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:20,000, dot blot: 1:80,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

technique(s)

direct ELISA: 1:7,000

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

species reactivity

bovine

species reactivity

goat

species reactivity

bovine

species reactivity

-

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000540015
0000537342
0000521301
0000537342
0000521301

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Related Content

Data Sheet - A0705

Instructions

Global Trade Item Number

SKUGTIN
A0705-1ML04061833334270
A0705-.25ML04061837513756

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