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A2064

抗ヒトIgG−アルカリフォスファターゼ抗体, マウスモノクロナール

clone GG-5, purified from hybridoma cell culture

別名:

モノクロナール抗ヒトIgG (γ-鎖特異的)

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あなたへ/カタログ番号在庫状況単価
0.2 mL
カートの在庫状況を確認する
¥40,500
1 mL
カートの在庫状況を確認する
¥133,000

この商品について

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
alkaline phosphatase conjugate
Clone:
GG-5, monoclonal
Application:
DB, ELISA (d), IHC, WB CL
Citations:
13

¥40,500


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biological source

mouse

Quality Segment

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

GG-5, monoclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

direct ELISA: 1:50,000, dot blot: 1:80,000, immunohistochemistry: suitable, western blot (chemiluminescent): 1:80,000

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

ヒトIgGと結合します。その他のヒトIg分子とは結合しません。抗体はヒトIgGのH鎖上の決定基に特異的です。本抗体は、3% PEG6000存在下の液層で抗原-抗体複合体を形成します。
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Monoclonal Anti-Human IgG (Γ-chain specific) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Application

液体沈降(Lppt)に適用。
Anti-Human IgG- Alkaline Phosphatase antibody, Mouse monoclonal has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • western blotting
  • dot blot
  • immunohistology

Elisa assays were performed using alkaline phosphatase conjugated monoclonal mouse anti-human IgG to detect antibodies against various S. Aureus antigens in the serum from healthy individuals. The antibody was diluted 1:30000 in PBSt and incubated with samples for 2 hours at 37 degrees. Reactin was developed using p-nitrophenyl phosphate substrate (Sigma).

Physical form

0.05M Trisバッファ-溶液 (pH 8.0, 1mM MgCl2, 1%BSA, 50%グリセロ-ル, 15mMアジ化ナトリウム含有)。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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当該品目
A3076A2189A9544
description

clone GG-5, purified from hybridoma cell culture

description

clone GE-1, purified from hybridoma cell culture

description

clone MB-11, purified from hybridoma cell culture

description

affinity isolated antibody, buffered aqueous solution

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

-

technique(s)

direct ELISA: 1:50,000, immunohistochemistry: suitable, dot blot: 1:80,000, western blot (chemiluminescent): 1:80,000

technique(s)

direct ELISA: 1:6,000, dot blot: 1:1,000

technique(s)

direct ELISA: 1:35,000, dot blot: 1:20,000 using 20 ng of human IgM/dot, dot blot: 1:30,000 using 20 ng of human IgM/dot (chemiluminescence)

technique(s)

direct ELISA: 1:55,000, dot blot: 1:40,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

biological source

mouse

biological source

mouse

biological source

mouse

biological source

goat

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

isotype

IgG1

isotype

IgG2b

isotype

IgG2b

isotype

-


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保管分類

10 - Combustible liquids

wgk

WGK 2

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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