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A3553

Acrylamide

suitable for electrophoresis, ≥99% (HPLC), powder

Synonym(s):

2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide

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100 g

Available to ship TODAYfromMILWAUKEE

$88.00
500 g

Available to ship TODAYfromMILWAUKEE

$307.00
1 kg

Available to ship TODAYfromMILWAUKEE

$460.00

About This Item

Linear Formula:
CH2=CHCONH2
CAS Number:
Molecular Weight:
71.08
NACRES:
NB.22
PubChem Substance ID:
eCl@ss:
39031205
UNSPSC Code:
41105319
EC Number:
201-173-7
MDL number:
Beilstein/REAXYS Number:
605349

$88.00


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vapor density

2.45 (vs air)

Quality Level

vapor pressure

0.03 mmHg ( 40 °C)

assay

≥99% (HPLC)

form

powder

technique(s)

electrophoresis: suitable

impurities

≤0.005% Acrylic acid, ≤0.005% Insoluble in water

conductivity

≤10 μmho (35% solution)

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

cation traces

Fe: ≤1 ppm, Mg: ≤3 ppm, heavy metals (as Pb): ≤5 ppm

SMILES string

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

General description

Acrylamide functions as a quencher of tryptophan fluorescence. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules. Bis-acrylamide is essential for crosslinking and acrylamide is required for the polymerization of monomers. Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis.

Application

Acrylamide has been used in the preparation of polyacrylamide gels.[1]

Other Notes

This substance has been tested against several types of hand protection for CE compliance. See Related Products to find the recommended gloves for handling this product.

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This Item
A888701696A9099
form

powder

form

crystals

form

powder blend

form

crystals

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

impurities

≤0.005% Acrylic acid, ≤0.005% Insoluble in water

impurities

-

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

impurities

≤0.005% Acrylic acid content, ≤0.005% Water insolubles

solubility

water: soluble 2.5 g/10 mL, clear, colorless

solubility

water: soluble 2.5 g/10 mL, clear, colorless

solubility

H2O: 0.25 g/mL at 20 °C, clear, colorless

solubility

water: soluble 2.5 g/10 mL, clear, colorless

mp

82-86 °C (lit.)

mp

82-86 °C (lit.)

mp

82-86 °C (lit.)

mp

82-86 °C (lit.)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


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pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

280.4 °F - closed cup

flash_point_c

138 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges



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Related Content

Product Information Sheet


The Protein Protocols Handbook (2007)
Relationships among cell morphology, intrinsic cell stiffness and cell-substrate interactions
Chiang MYM, et al.
Biomaterials, 34(38), 9754-9762 (2013)
Chemistry, biochemistry, and safety of acrylamide. A review
Friedman M
Journal of Agricultural and Food Chemistry, 51(16), 4504-4526 (2003)



Global Trade Item Number

SKUGTIN
A3553-500G04061835542048
A3553-1KG04061835542031
A3553-100G04061835542024

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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