The concentration of this product varies from lot to lot and may range from 2.0 - 5.0 mg/mL. This lot-specific concentration if reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/b8895#product-documentation
B8895
Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|---|---|
0.5 mL | Available to ship TODAYfromMILWAUKEE | $157.00 |
1 mL | Available to ship TODAYfromMILWAUKEE | $257.00 |
2 mL | Available to ship TODAYfromMILWAUKEE | $395.00 |
About This Item
Conjugate:
biotin conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB
Citations:
86
biological source
goat
Quality Segment
conjugate
biotin conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:200,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800, western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)
storage temp.
−20°C
target post-translational modification
unmodified
General description
IgG antibodies regulate several functions such as phagocytosis and complement activation. Thus they play a crucial role in facilitating cytological immune responses. Anti-rabbit IgG (whole molecule)–biotin antibody can be used in immunohistochemistry. The product shows minimal interspecies cross reactivity to human IgG hence can be used in enzyme immunoassays or dot blot in presence of human IgG. Anti-rabbit IgG (whole molecule)–biotin antibody reacts specifically to all rabbit immunoglobulin but has no reactivity with human IgG.
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule)-Biotin antibody reacts with rabbit serum and rabbit IgG. The product does not cross-react with human IgG.
Anti-Rabbit IgG (whole molecule)-Biotin antibody reacts with rabbit serum and rabbit IgG. The product does not cross-react with human IgG.
Immunogen
Purified rabbit IgG
Application
Biochem/physiol Actions
Avidin, Streptavidin or ExtrAvidine show strong affinity interaction with biotin rendering the biotin-avidin system an extremely effective tool in molecular biology, protein chemistry and immunology. The high specificity of the biotinylated antibody to rabbit IgG and the stability of the biotin-avidin complex has been exploited in variety of reagents (Avidin, Streptavidin or ExtrAvidin conjugated to FITC, TRITC, Peroxidase or Alkaline Phosphatase), for the detection and quantitation of rabbit IgG.
Binds all rabbit Igs.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide
Preparation Note
Adsorbed to reduce background staining with human samples.
Other Notes
Antibody adsorbed with human IgG.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description affinity isolated antibody, buffered aqueous solution | description affinity isolated antibody | description whole antiserum, lyophilized powder | description affinity isolated antibody, buffered aqueous solution |
| technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800, western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well), direct ELISA: 1:200,000 | technique(s) western blot (chemiluminescent): 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg/mL), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, direct ELISA: 1:30,000 using using 5 μg/ml of rabbit IgG for the coating and OPD substrate | technique(s) radioimmunoassay: suitable | technique(s) direct ELISA: 1:45,000, western blot: 1:100,000-1:200,000 using detecting actin in total cell extract of HeLa cells (5-10 μg per well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form whole antiserum | antibody form affinity isolated antibody |
| biological source goat | biological source goat | biological source goat | biological source goat |
| conjugate biotin conjugate | conjugate peroxidase conjugate | conjugate unconjugated | conjugate alkaline phosphatase conjugate |
| form buffered aqueous solution | form - | form lyophilized powder | form buffered aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
Still not finding the right product?
Explore all of our products under Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat
— or —
Try our Product Selector Tool to narrow your options
Storage Class
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
what is the concentration of this antibody?
1 answer-
Helpful?
-