Yes, as mentioned in the product datasheet, the powder form should be stored at –20°C. Stock solutions may be prepared at 100 mg/mL in DMEM/F12 or other culture medium, and stored at –20°C in frozen aliquots. Avoid repeated freeze/thaw cycles. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/173/127/c2139pis.pdf
C2139
Collagenase from Clostridium histolyticum
suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV), Type VIII, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid
Collagenase from Clostridium histolyticum
Synonym(s):
Clostridiopeptidase A
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 mg | Available to ship TODAYfromMILWAUKEE | $137.00 | |
| 500 mg | Available to ship TODAYfromMILWAUKEE | $398.00 | |
| 1 g | Available to ship TODAYfromMILWAUKEE | $687.00 | |
| 5 g | Available to ship TODAYfromMILWAUKEE | $2,900.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-582-9
MDL number:
Specific activity:
≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid
Biological source:
Clostridium histolyticum
biological source
Clostridium histolyticum
Quality Level
type
Type VIII
form
powder
specific activity
≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid
mol wt
68-125 kDa
suitability
suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV)
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
General description
Clostridium histolyticum is a pathogenic clostridium that produces crude collagenases. This is a mixture of enzymes containing collagenase, non-specific proteases and clostripain.[1]
Application
Collagenase may be used:[1]
- for the preparation of arterial tissue for the study of advanced glycosylation end products (AGE)
- for use along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues.
- in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes.
- for the preparation of viable hepatocytes from rat liver and for the isolation of fat cells from rat adipose tissue
Biochem/physiol Actions
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(β-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, β-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline. Collagenase enzymes and neutral protease plays an important role in the effective release of cells from tissue. Collagenase recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X represents a neutral amino acid.
Analysis Note
Also contains clostripain, nonspecific neutral protease and tryptic activities.
Other Notes
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.
1 of 1
This Item | |||
|---|---|---|---|
| biological source Clostridium histolyticum | biological source Clostridium histolyticum | biological source - | biological source - |
| specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid | specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid | specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid | specific activity ≥1 FALGPA units/mg solid, >125 CDU/mg solid |
| suitability suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV) | suitability - | suitability suitable for release of physiologically active rat hepatocytes | suitability - |
| form powder | form powder | form powder | form powder |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Still not finding the right product?
Explore all of our products under Collagenase from Clostridium histolyticum
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
Related Content
Instructions
Ce Tang et al.
Nature immunology, 19(7), 755-765 (2018-06-20)
The cytokines IL-17A and IL-17F have 50% amino-acid identity and bind the same receptor; however, their functional differences have remained obscure. Here we found that Il17f-/- mice resisted chemically induced colitis, but Il17a-/- mice did not, and that Il17f-/- CD45RBhiCD4+
Fleischer, S. and B. Fleischer
Methods in Enzymology, 192, 829-829 (1990)
Parker, L.
Methods in Enzymology, 190, 480-480 (1990)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C2139-1G | 04061833475188 |
| C2139-5G | 04061833475201 |
| C2139-100MG | 04061833475171 |
| C2139-500MG | 04061833475195 |
-
Can I use C2139 to make collagenase solution with medium, and then store it at -20?
1 answer-
Helpful?
-
-
is C2139 sterile ?
1 answer-
Product C2139 is not sterile.
Helpful?
-