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D4309

REDTaq® DNA Polymerase

Taq for routine PCR with inert dye, 10X buffer included

Sinonimo/i:

DNA polymerase with loading dye, Taq DNA polymerase

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Taglio della confezioneSKUDisponibilitàPrezzo
50 units
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CHF 58.30
250 units
Per conoscere la disponibilità, visualizza il carrello
CHF 260.00
1000 units
Per conoscere la disponibilità, visualizza il carrello
CHF 969.00

Informazioni su questo articolo

UNSPSC Code:
12352204
NACRES:
NA.55
MDL number:
MFCD01635818
Concentration:
1 unit/μL

CHF 58.30

Per conoscere la disponibilità, visualizza il carrello
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Quality Level

200

form

liquid

usage

sufficient for 1000 reactions, sufficient for 250 reactions, sufficient for 50 reactions

feature

dNTPs included: no, hotstart: no

concentration

1 unit/μL

technique(s)

PCR: suitable

color

red

input

purified DNA

suitability

suitable for PCR

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

General description

REDTaq® DNA Polymerase is a unique blend of Sigma′s quality Taq DNA Polymerase combined with an inert red dye. This dye enables quick visual confirmation of enzyme addition and reaction mixing. An aliquot of the samples (5-10 μl) can then be loaded directly onto an agarose gel for electrophoresis following PCR. The red dye migrates slightly faster than bromophenol blue at about the same rate as a 125 base pair fragment in a 1% agarose gel. Since no additional loading buffers are added to the reaction following PCR, reamplification is possible.
The red dye has no effect on automated or manual sequencing, restriction digestions or other downstream applications. However, if removing the dye is desired, this can easily be accomplished using any standard purification method.

Application

For routine PCR amplifications REDTaq® DNA Polymerase has been used in polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR) analysis.

Features and Benefits

  • Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications.
  • Visual confirmation that not only has the enzyme been added, but that proper mixing has occurred.
  • No additional loading dyes are necessary. An aliquot can be taken directly from the reaction and loaded onto an agarose gel for electrophoresis.

Packaging

Provided with 10X reaction buffer containing MgCl2

Other Notes

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

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REDTaq® DNA Polymerase Taq for routine PCR with inert dye, 10X buffer included

Sigma-Aldrich

D4309

REDTaq® DNA Polymerase

REDTaq® Genomic DNA Polymerase without MgCl2

Sigma-Aldrich

D2812

REDTaq® Genomic DNA Polymerase

REDTaq® SuperPak™ DNA Polymerase Taq for routine PCR with inert dye; with 10X buffer & dNTP mix

Sigma-Aldrich

D6063

REDTaq® SuperPak™ DNA Polymerase

JumpStart™ REDTaq® DNA Polymerase Hot-start Taq enzyme with inert dye, 10X buffer included

Sigma-Aldrich

D8187

JumpStart™ REDTaq® DNA Polymerase

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

form

liquid

form

liquid

form

liquid

form

liquid

concentration

1 unit/μL

concentration

1 unit/μL

concentration

1 unit/μL

concentration

1 unit/μL

suitability

suitable for PCR

suitability

-

suitability

suitable for PCR

suitability

suitable for PCR

application(s)

agriculture

application(s)

agriculture

application(s)

-

application(s)

-

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Classe di stoccaggio

10 - Combustible liquids

ppe

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000554807
0000554807
0000420518
0000420518
0000340845

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Protocolli

Applications with REDTaq™

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

Standard PCR Protocol

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

Protocollo PCR standard

Imparate le fasi del protocollo PCR standard e rivedete gli elenchi dei reagenti o i parametri di ciclaggio. Questo metodo per l'amplificazione PCR di routine del DNA utilizza la Taq DNA polimerasi standard.

Visualizza tutti i protocolli

Articoli

Introduction & Historical Timelines

Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

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Chen H, et al.
Biochemical and Biophysical Research Communications, 369(3), 801-806 (2008)
Mutation or polymorphism of the CD20 gene is not associated with the response to R-CHOP in diffuse large B cell lymphoma patients
Sar A, et al.
Leukemia Research, 33(6), 792-797 (2009)
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Numero articolo commerciale globale

SKUGTIN
D4309-50UN04061833564301
D4309-1KU04061835572632
D4309-250UN04061835572649

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