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D8537

Dulbecco’s Phosphate Buffered Saline

liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Dulbecco’s Phosphate Buffered Saline
1 of 6 reviewers took part in a promotion

Synonym(s):

DPBS

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Pack SizeSKUAvailabilityPrice
100 mL
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$18.90
500 mL
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$35.20
1 L
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$69.70
6 x 500 mL
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$195.00
6 x 1 L
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$370.00
24 x 500 mL
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$703.00

About This Item

UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.75
MDL number:

$18.90


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Product Name

Dulbecco′s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: no (Dextro)
sodium pyruvate: no

shipped in

ambient

storage temp.

room temp

General description

Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS buffer is used to irrigate, wash, and dilute mammalian cells while maintaining osmotic balance. It provides water and inorganic ions essential for normal cell metabolism. DPBS with calcium and magnesium facilitates cell binding and clumping. Dulbecco′s PBS without these ions can be used to wash and rinse suspended cells. Phosphate buffering maintains the pH in the physiological range.

Application

Dulbecco′s Phosphate Buffered Saline has been used:

  • for the dilution of fresh red blood cells to study the trapping of red blood cell aggregate.
  • to wash the wells of a microtiter plate.[1]
  • to wash the cells before resuspending them in lysis buffer for DNA isolation.[2]
  • as a component of embryo holding medium for vitrification of embryos.

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This Item
59321C59331C59300C
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

300

Quality Level

400

Quality Level

400

Quality Level

500

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

room temp

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

impurities

endotoxin, tested

impurities

-

impurities

-

impurities

-


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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Protocols

How to stain organoids? A complete step-by-step protocol for immunofluorescent (IF) and immunocytochemical (ICC) staining of organoid cultures using antibodies

Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.

Technical information for working with human skeletal muscle cells including thawing, subculturing and cryopreservation

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Applied and environmental microbiology, 79(16), 4951-4957 (2013-06-19)
The genus Arcobacter is composed of 17 species which have been isolated from various sources. Of particular interest are A. butzleri, A. cryaerophilus, and A. skirrowii, as these have been associated with human cases of diarrhea, the probable transmission routes
Elliptical optical tweezers for trapping a red blood cell aggregate
Kauppila AJ
Photonics Letters of Poland, 3(3), 95-97 (2011)
Cameron Goertzen et al.
Biological procedures online, 20, 20-20 (2018-10-26)
Invadopodia are actin-rich, proteolytic structures that enable cancer cell to invade into the surrounding tissues. Several in vitro invasion assays have been used in the literature ranging from directional quantitative assays to complex three-dimensional (3D) analyses. One of the main



Global Trade Item Number

SKUGTIN
D8537-6X500ML04061838259400
D8537-24X500ML04061833589731
D8537-100ML04061833589724
D8537-1L04061838259370
D8537-6X1L04061838259394
D8537-500ML04061838259387

Questions

1–10 of 11 Questions  
  1. Eu gostaria de saber a osmolaridade da solução de DMPBS.

    1 answer
    1. This product has an osmolarity range of 275–304 mOsm/kg.

      Helpful?

  2. Quería saber si éste producto se puede considerar RNAsa free para realizar durante la hibridación in situ

    1 answer
    1. This product is a sterile-filtered M Quality Level 300 product. It is not molecular biology grade and does not have specifications such as RNase and DNase free. Therefore, this product cannot be considered RNase free for use in in situ hybridization.

      Helpful?

  3. Bonjour, pourriez-vous m'indiquer le numéro de CAS de la solution de phosphate saline dulbecco sous forme liquide

    1 answer
    1. This item is a multi-component product. A CAS number cannot be applied to this type of product. The Safety Data Sheet document includes the individual CAS numbers of those components that may be potentially hazardous.

      Helpful?

  4. Hi, is this product GMP-grade or are GMP-garde material is used for the production of this?

    1 answer
    1. This product is not GMP-grade. Unfortunately. a GMP-grade version of this product is not available.

      Helpful?

  5. What is the concentration of the product in Molarity?

    1 answer
    1. This is a multi-component formulation. A 'molarity' is not reported for this type of product. See below for the list of components:
      Product D8537 contains:
      Potassium chloride: 0.2 g/l
      Potassium phosphate monobasic: 0.2 g/l
      Sodium chloride: 8 g/l
      Sodium Phosphate dibasic: 1.15 g/l

      Please see the link below for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/124/464/d8537for.pdf

      Helpful?

  6. What is the bottle thread type on the 1L bottle (D8537-1L)

    1 answer
    1. The product is packaged in a square PET bottle with an HDPE cap. The cap specifications are as follows:
      Thread Type 38-430
      Thread Diameter 37.3 mm

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. What is the formulation for Product D8537, Dulbecco's Phosphate?

    1 answer
    1. The formulation for 1 liter is 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride.

      Helpful?

  9. Is Product D8537, Dulbecco's Phosphate, a 1X or 10 X concentration?

    1 answer
    1. This is a 1X concentration. The 10X concentration is listed under product D1408.

      Helpful?

  10. What is the pH of a solution of Product D8537, Dulbecco's Phosphate?

    1 answer
    1. The pH will be in the range of 7.1-7.5, according to the specification sheet.

      Helpful?

1–10 of 11 Questions  

Reviews

1 of 6 reviewers took part in a promotion

Active Filters

  1. Atlanta, GA
    • Reviews 3
    • Votes 95
    5 out of 5 stars.

    All-purpose DPBS

    It's DPBS with calcium-magnesium modification. Worked pretty well for me for various applications.

    Helpful?

  2. 5 Ratings-Only Reviews