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D8906

Dextran sulfate sodium salt from Leuconostoc spp.

Molecular Biology, average Mw >500,000 (dextran starting material), contains 0.5-2% phosphate buffer

Synonym(s):

6-O-(6-O-beta-D-Glucopyranosyl-beta-D-glucopyranosyl)-D-glucose, Manninotriose

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About This Item

CAS Number:
9011-18-1
UNSPSC Code:
12352201
NACRES:
NA.31
MDL number:
MFCD00081551

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biological source

synthetic

Quality Level

300

grade

Molecular Biology

form

powder

mol wt

average Mw >500,000 (dextran starting material)

contains

0.5-2% phosphate buffer

composition

Sulfur Content, 16.0-19.0%

technique(s)

FISH: suitable, FTIR: suitable

color

off-white

useful pH range

6.0-8 (10 g/L)

solubility

H2O: 100 mg/mL

suitability

suitable for marker for electrophoresis, suitable for nucleic acid hybridization

application(s)

genomic analysis
sample preparation

General description

Dextran sulfate sodium (DSS) is a synthetic sulfated derivative of dextran, a complex polysaccharide composed of glucose molecules. DSS is characterized as a biocompatible, highly branched polysaccharide with 1–6 and 1–4 glycosidic linkages, featuring approximately 2.3 sulfate groups per glucosyl unit. Dextran sulfate is a sulfated polysaccharide with a variable molecular weight ranging from 5 to 1,400 kDa. It finds extensive use in molecular biology and biochemical research, with a particular emphasis on gastrointestinal investigations.DSS is widely recognized for its ability to induce colonic inflammation in mouse models of inflammatory bowel disease (IBD). It is widely recognized for its capacity to induce colonic inflammation in mouse models of inflammatory bowel disease (IBD) by creating models of acute, chronic, and relapsing IBD, by adjusting its concentration and administration frequency. Furthermore, Dextran sulfate is applied in various molecular biology techniques, such as precipitating lipoproteins, accelerating DNA probe hybridization, and facilitating ribosomal isolations. Notably, DSS serves as a valuable cross-linker for chitosan, allowing for the creation of microparticles and nanoparticles used in controlled drug delivery systems. Beyond these applications, DSS plays a central role in diverse research areas, particularly immunology and virology, where it actively modulates immune responses, enhances humoral immunity, influences B-lymphocyte activation, and affects thymocyte reactivity to lectins.

Application

Dextran sulfate has many applications and when performing nucleic acid hybridizations, may be used as an accelerant to decrease the time required for probe annealing. Dextran sulfate may be included in the hybridization buffer when the rate of hybridization is a limiting factor in detecting rare sequences or it may be included in the hybridization buffer when a limited amount of probe is available. Dextran sulfate functions as an accelerant by excluding the nucleic acid from the volume of solution occupied by the polymer, thereby, increasing the effective probe concentration.
Tested for suitability in nucleic acid hybridizations
Dextran sulfate has been used

  • as a molecular weight marker to assess the purity and homogeneity of the purified polysaccharides by gel electrophoresis
  • as a component of the hybridization buffer in fluorescence in situ hybridization (FISH) protocol for DNA detection and analysis[1]

Biochem/physiol Actions

Dextran sulfate, being a sulfated polysaccharide, consistently triggers experimental acute and chronic colitis. It is believed to initiate mucosal injury and inflammation, primarily through a direct toxic impact on epithelial cells. This initial effect activates macrophages and T lymphocytes, ultimately leading to cytokine-mediated cytotoxicity.

Features and Benefits

  • Suitable for Nucleic acid hybridization & Molecular Biology
  • Dextran Sulfate with an average molecular weight of >500,000
  • Sulfur Content of 16.0-19.0 %
  • Versatile and adaptable for wide variety of laboratory and research applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

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Dextran sulfate sodium salt from Leuconostoc spp. Molecular Biology, average Mw >500,000 (dextran starting material), contains 0.5-2% phosphate buffer

Sigma-Aldrich

D8906

Dextran sulfate sodium salt from Leuconostoc spp.

Dextran sulfate sodium salt from Leuconostoc spp. low sulfate content, Mr ~40,000

Sigma-Aldrich

52423

Dextran sulfate sodium salt from Leuconostoc spp.

Dextran sulfate sodium salt from Leuconostoc spp. mol wt 6,500-10,000 by GPC

Sigma-Aldrich

D4911

Dextran sulfate sodium salt from Leuconostoc spp.

Dextran sulfate sodium salt from Leuconostoc spp. avg mol wt >500,000 (dextran starting material)

Sigma-Aldrich

D8787

Dextran sulfate sodium salt from Leuconostoc spp.

technique(s)

FISH: suitable, FTIR: suitable

technique(s)

-

technique(s)

-

technique(s)

-

biological source

synthetic

biological source

(Leuconostoc spp)

biological source

bacterial (Leuconostoc)

biological source

bacterial (Leuconostoc)

grade

Molecular Biology

grade

-

grade

-

grade

-

solubility

H2O: 100 mg/mL

solubility

H2O: 100 mg/mL, clear, colorless to faintly greenish-yellow

solubility

H2O: 100 mg/mL

solubility

H2O: 100 mg/mL

Quality Level

300

Quality Level

100

Quality Level

100

Quality Level

200

form

powder

form

powder

form

powder

form

powder or crystals

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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000459172
0000533816
0000529831
0000529831
0000459172

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Protocols

Dextran Sulfate

Dextran sulfates as sodium salts are water-soluble and stable, containing approximately 17% sulfur for various applications.

Articles

Fluorescent in situ Hybridization (FISH)

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

Related Content

Dextran and Polysaccharides Overview

Dextrans are polysaccharides with molecular weights ≥1,000 Dalton, featuring a linear backbone of α-linked d-glucopyranosyl repeating units.

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Global Trade Item Number

SKUGTIN
D8906-500G04061835544240
D8906-5G04061835544264
D8906-10G04061835506262
D8906-100G04061835544233
D8906-50G04061835544257

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      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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