All Photos(4)

D8906

Sigma-Aldrich

Dextran sulfate sodium salt from Leuconostoc spp.

for molecular biology, average Mw >500,000 (dextran starting material), contains 0.5-2% phosphate buffer

CAS Number:
MDL number:
NACRES:
NA.31

Quality Level

biological source

synthetic

grade

for molecular biology

form

powder

mol wt

average Mw >500,000 (dextran starting material)

contains

0.5-2% phosphate buffer

composition

Sulfur Content, 16.0-19.0%

solubility

H2O: 100 mg/mL

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

Dextran sulfates are supplied as the sodium salt forms that are soluble and stable in water. It consist of 17% (approx.) sulfur equivalent to 2.3 (approx.) sulfate groups per glucosyl residue. Dextran is a (complex branched glucan) polymer of anhydroglucose. It is composed of approximately 95% α-D-(166) linkages and the remaining (163) linkages account for the branching of dextran.

Application

Dextran sulfate has many applications and when performing nucleic acid hybridizations, may be used as an accelerant to decrease the time required for probe annealing. Dextran sulfate may be included in the hybridization buffer when the rate of hybridization is a limiting factor in detecting rare sequences or it may be included in the hybridization buffer when a limited amount of probe is available. Dextran sulfate functions as an accelerant by excluding the nucleic acid from the volume of solution occupied by the polymer, thereby, increasing the effective probe concentration.

Packaging

5, 10, 50, 100, 500 g in poly bottle

Suitability

Tested for suitability in nucleic acid hybridizations

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Benjamin R King et al.
Journal of virology, 92(12) (2018-04-13)
Lymphocytic choriomeningitis mammarenavirus (LCMV) is an enveloped, negative-strand RNA virus that causes serious disease in humans but establishes an asymptomatic, lifelong infection in reservoir rodents. Different models have been proposed to describe how arenaviruses regulate the replication and transcription of...
Robert N Duncan et al.
PloS one, 10(12), e0145810-e0145810 (2015-12-30)
Wnt signaling regulates multiple aspects of vertebrate central nervous system (CNS) development, including neurogenesis. However, vertebrate genomes can contain up to 25 Wnt genes, the functions of which are poorly characterized partly due to redundancy in their expression. To identify...
D Huber et al.
Biomicrofluidics, 12(4), 042212-042212 (2018-06-12)
This paper describes a micro fluorescence in situ hybridization (μFISH)-based rapid detection of cytogenetic biomarkers on formalin-fixed paraffin embedded (FFPE) tissue sections. We demonstrated this method in the context of detecting human epidermal growth factor 2 (HER2) in breast tissue...
Danielle L Cullinane et al.
Reproduction (Cambridge, England), 149(1), 43-54 (2014-10-23)
The protamine 1 (Prm1) and sperm mitochondria-associated, cysteine-rich protein (Smcp) mRNAs exemplify a widespread pattern of mRNA-specific regulation of mRNA translation in post-meiotic spermatogenic cells, spermatids. Both mRNAs are transcribed and initially stored in free-mRNPs in early spermatids, and translated...
Bhavana Tiwari et al.
Current biology : CB, 27(19), 3010-3016 (2017-10-03)
Retrotransposons are a pervasive class of mobile elements present in the genomes of virtually all forms of life [1, 2]. In metazoans, these are preferentially active in the germline, which, in turn, mounts defenses that restrain their activity [3, 4]....

Articles

Fluorescent in situ Hybridization (FISH)

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

Protocols

Dextran Sulfate

Dextran sulfates are supplied as the sodium salt forms, making them soluble and stable in water. Dextran sulfate contains approximately 17% sulfur which is equivalent to approximately 2.3 sulfate groups per glucosyl residue.

Related Content

Dextran and Related Polysaccharides

Learn about C6H10O6 or dextran formation, classes and naming from MilliporeSigma. Dextrans are polysaccharides with molecular weights ≥1,000 Dalton, which have a linear backbone of α-linked d-glucopyranosyl repeating units.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service