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DEAE–Sepharose^™

Name: DEAE–Sepharose<SUP>™</SUP>
Brand: SIGMA
Price: 290 USD

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Synonym(s):

Diethylaminoethyl–Sepharose^™

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Pack Size	SKU	Availability	Price
50 mL		Check Cart for Availability	$290.00
100 mL		Check Cart for Availability	$457.00

About This Item

CAS Number:

57407-08-6

UNSPSC Code:

47101511

NACRES:

NA.56

MDL number:

MFCD00146630

$290.00

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Properties

Quality Level

200

form

suspension

technique(s)

affinity chromatography: suitable

matrix

6% cross-linked agarose

bead size

45-165 μm (wet)

pore size

~4,000,000 Da exclusion limit

pH

2—12

capacity

110-160 μeq/mL binding capacity(gel volume)

Description

General description

DFF100-500Ml′s update product number is GE17-0709-01

Application

DEAE-Sepharose^™ is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose^™ has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile.

DEAE-Sepharose has been used in:

anion exchange chromatography
to screen polyisoprenyl phosphate phosphatase activity[1]
to purify isoinhibitors
for the purification of human immunodeficiency virus (HIV)
glycoprotein envelope (gp140 env)

Biochem/physiol Actions

Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose.

Legal Information

Sepharose is a trademark of Cytiva

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1 of 1

This Item	A50120	DCL6B100	CCF100
Sigma-Aldrich DFF100 DEAE–Sepharose^™	Sigma-Aldrich A50120 DEAE–Sephadex^®	Sigma-Aldrich DCL6B100 DEAE–Sepharose^™	Sigma-Aldrich CCF100 CM Sepharose^™
matrix 6% cross-linked agarose	matrix cross-linked dextran	matrix 6% cross-linked agarose	matrix 6% cross-linked agarose
pore size ~4,000,000 Da exclusion limit	pore size ~200,000 Da exclusion limit	pore size ~4,000,000 Da exclusion limit	pore size 4,000,000 exclusion limit (av. mol. wt.)
capacity 110-160 μeq/mL binding capacity(gel volume)	capacity 3-4 meq/g binding capacity	capacity 130-170 μeq/mL binding capacity (gel volume)(gel volume)	capacity 90-130 μeq/mL(gel basis)
form suspension	form powder	form suspension	form suspension
technique(s) affinity chromatography: suitable	technique(s) protein purification: suitable	technique(s) affinity chromatography: suitable	technique(s) affinity chromatography: suitable
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

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pictograms

GHS02

signalword

Warning

hcodes

H226

pcodes

P210 - P233 - P240 - P241 - P242 - P243

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

100.4 - 109.4 °F

flash_point_c

38 - 43 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Global Trade Item Number

SKU	GTIN
DFF100-100ML	04061833591550
DFF100-50ML	04061833591567

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