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Endoglycosidase F3 from Elizabethkingia miricola

Name: Endoglycosidase F3 from <I>Elizabethkingia miricola</I>
Brand: SIGMA

recombinant, expressed in E. coli, 30 U/mg

Synonym(s):

Elizabethkingia miricola, Endo-β-N-acetylglucosaminidase F3, Endoglycosidase F3 from Elizabethkingia (Chryseobacterium/Flavobacterium) meningosepticum

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

3.2.1.96 (BRENDA, IUBMB)

MDL number:

MFCD00146843

Specific activity:

30 U/mg

Recombinant:

expressed in E. coli

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Properties

recombinant

expressed in E. coli

Quality Level

200

conjugate

(N-linked)

form

solution

specific activity

30 U/mg

mol wt

32 kDa

shipped in

wet ice

storage temp.

2-8°C

Description

Application

Endoglycosidase F3 from Elizabethkingia miricola has been used to analyze core fucosylation and tryptic digests of serum proteins.

Biochem/physiol Actions

Cleaves asparagine-linked biantennary and triantennary complex, oligosaccharides depending on the state of core fucosylation and peptide linkage.

Endoglycosidase F3 belongs to the glycoside hydrolase family 18 (GH18). It has hydrolytic activity. Endoglycosidase F3 glycosylates α-1,6-fucosylated GlcNAc derivative to give natural, core fucosylated complex-type N-glycopeptides.

Packaging

Supplied with 5× Reaction Buffer, 250 mM sodium acetate, pH 4.5

Physical form

Aseptically filled solution in 20 mM Tris-HCl, pH 7.5

Other Notes

One unit will release N-linked oligosaccharides from 1 μmole of denatured porcine fibrinogen in 1 minute at 37 °C, pH 4.5.

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This Item	E0639	E9762	324727
Sigma-Aldrich E2264 Endoglycosidase F3 from Elizabethkingia miricola	Sigma-Aldrich E0639 Endoglycosidase F2 from Elizabethkingia miricola	Sigma-Aldrich E9762 Endoglycosidase F1 from Elizabethkingia miricola	Sigma-Aldrich 324727 Endoglycosidase F3, Elizabethkingia meningosepticum, Recombinant, E. coli
specific activity 30 U/mg	specific activity 20 U/mg	specific activity ≥16 U/mg	specific activity ≥30 units/mg protein, ≥5 units/mL
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
form solution	form solution	form buffered aqueous solution	form liquid
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
mol wt 32 kDa	mol wt 32 kDa	mol wt 32 kDa	mol wt -

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Global Trade Item Number

SKU	GTIN
E2264-0.1UN	04061832856391
E2264-.2UN	04061833602560

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